Anticorps Polyclonal de lapin anti-PPP2R2B/A/C/D

PPP2R2B/A/C/D Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 13123-1-PBS

Synonymes

---

Galerie de données de validation

• 

  WB analysis using 13123-1-AP (same clone as 13123-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 13123-1-AP (PPP2R2B/A/C/D antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13123-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 13123-1-AP (same clone as 13123-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 13123-1-AP (PPP2R2B/A/C/D antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13123-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 13123-1-AP (same clone as 13123-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 13123-1-AP (PPP2R2B/A/C/D antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13123-1-PBS in a different storage buffer formulation.

• 

  WB analysis of MCF-7 using 13123-1-AP (same clone as 13123-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 13123-1-AP (PPP2R2B/A/C/D antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13123-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 13123-1-AP (same clone as 13123-1-PBS)

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 13123-1-AP (PPP2R2B/A/C/D antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13123-1-PBS in a different storage buffer formulation.

• 

  IP experiment of SH-SY5Y using 13123-1-AP (same clone as 13123-1-PBS)

  IP result of anti-PPP2R2B/A/C/D (IP:13123-1-AP, 4ug; Detection:13123-1-AP 1:500) with SH-SY5Y cells lysate 1200ug. This data was developed using the same antibody clone with 13123-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 13123-1-AP (same clone as 13123-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13123-1-AP (PPP2R2B/A/C/D antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13123-1-PBS in a different storage buffer formulation.

• 

  IF Staining of SH-SY5Y using 13123-1-AP (same clone as 13123-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using PPP2R2B/A/C/D antibody (13123-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13123-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

13123-1-PBS cible PPP2R2B/A/C/D dans les applications de WB, IHC, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PPP2R2B/A/C/D Protéine recombinante Ag3860
• Nom complet: protein phosphatase 2 (formerly 2A), regulatory subunit B, beta isoform
• Masse moléculaire calculée: 443 aa, 52 kDa
• Poids moléculaire observé: 52 kDa
• Numéro d’acquisition GenBank: BC031790
• Symbole du gène: PPP2R2B
• Identification du gène (NCBI): 5521
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

The PPP2R2B gene encodes a deduced 443-amino acid protein of approximately 52 kDa,which is a brain-specific regulatory subunit B of protein phosphatase 2. PPP2R2B is a Subunit of PP2A, a highly conserved constitutive enzyme(PMID:11719278). PPP2R2B (Bβ) is an important regulator of protein phosphatase 2A (PP2A) activity in the brain. Through differential promoter usage and alternative splicing, two major isoforms Bβ1 and Bβ2 with divergent sub-cellular targeting N termini are produced. Bβ plays an important role in neuronal survival. It has 5 isoforms produced by alternative splicing. This antibody has cross-reactivity to PPP2R2A, PPP2R2C and PPP2R2D.

{{ptg:RelatedPrimaryAntibodies}}