Anticorps Monoclonal anti-PPP3CB

• Phare
• Validé par KD/KO

PPP3CB Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

3C1A3

N° de cat : 66614-1-PBS

Synonymes

CALNA2, 3C1A3, Calmodulin-dependent calcineurin A subunit beta isoform, CALNB, CAM PRP catalytic subunit

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Galerie de données de validation

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  WB analysis using 66614-1-Ig (same clone as 66614-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66614-1-Ig (PPP3CB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66614-1-PBS in a different storage buffer formulation.

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  WB analysis of rat brain using 66614-1-Ig (same clone as 66614-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 66614-1-Ig (PPP3CB antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66614-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 66614-1-Ig (same clone as 66614-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 66614-1-Ig (PPP3CB antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66614-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 66614-1-Ig (same clone as 66614-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66614-1-Ig (PPP3CB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66614-1-PBS in a different storage buffer formulation.

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  WB analysis of fetal human brain using 66614-1-Ig (same clone as 66614-1-PBS)

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66614-1-Ig (PPP3CB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66614-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66614-1-Ig (same clone as 66614-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PPP3CB antibody (66614-1-Ig, Clone: 3C1A3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66614-1-PBS in a different storage buffer formulation.

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  IF Staining of U-251 using 66614-1-Ig (same clone as 66614-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using PPP3CB antibody (66614-1-Ig, Clone: 3C1A3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66614-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66614-1-PBS cible PPP3CB dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: PPP3CB Protéine recombinante Ag26987
• Nom complet: protein phosphatase 3 (formerly 2B), catalytic subunit, beta isoform
• Masse moléculaire calculée: 525 aa, 59 kDa
• Poids moléculaire observé: 59 kDa
• Numéro d’acquisition GenBank: BC028049
• Symbole du gène: PPP3CB
• Identification du gène (NCBI): 5532
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

PPP3CB is one of the three isoforms of the A subunit of calcineurin (CnA). Calcineurin is a calcium-dependent serine/threonine phosphatase. It consists of two subunits: subunit A (CnA) and subunit B (CnB). For CnA, three isoforms PPP3CA (CnAα), PPP3CB (CnAβ) and PPP3CC (CnAγ) have been identified. PPP3CB is a calcium-dependent, calmodulin-stimulated protein phosphatase. It may have a role in the calmodulin activation of calcineurin.

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