Anticorps Polyclonal de lapin anti-PPP3R1

PPP3R1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(7)

N° de cat : 13210-1-AP

Synonymes

Calcineurin subunit B type 1, CALNB1, CNA2, CNB, CNB1

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Galerie de données de validation

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  WB analysis of K-562 using 13210-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 13210-1-AP (PPP3R1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 13210-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 13210-1-AP (PPP3R1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human stomach using 13210-1-AP

  human stomach tissue were subjected to SDS PAGE followed by western blot with 13210-1-AP (PPP3R1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 13210-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13210-1-AP (PPP3R1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 13210-1-AP

  IP result of anti-PPP3R1 (IP:13210-1-AP, 3ug; Detection:13210-1-AP 1:300) with HeLa cells lysate 5000ug.

• 

  IHC staining of mouse testis using 13210-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13210-1-AP (PPP3R1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 13210-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13210-1-AP (PPP3R1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 13210-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 13210-1-AP (PPP3R1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 13210-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 13210-1-AP (PPP3R1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 13210-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PPP3R1 antibody (13210-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, cellules HeLa, cellules K-562, tissu cérébral de rat, tissu d'estomac humain
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu testiculaire de souris, tissu cérébral humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 6 publications below
• IF: See 1 publications below

Informations sur le produit

13210-1-AP cible PPP3R1 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, poulet, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PPP3R1 Protéine recombinante Ag3992
• Nom complet: protein phosphatase 3 (formerly 2B), regulatory subunit B, alpha isoform
• Masse moléculaire calculée: 170 aa, 19 kDa
• Poids moléculaire observé: 19 kDa
• Numéro d’acquisition GenBank: BC027913
• Symbole du gène: PPP3R1
• Identification du gène (NCBI): 5534
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Protein phosphatase 3 (PPP3, formerly PP2B, Calcineurin), a serine/ threonine protein phosphatase, is a heterodimer composed of one catalytic subunit (PPP3C, Calcineurin A) and one regulatory subunit (PPP3R, Calcineurin B). PPP3R has two isoforms, PPP3R1 and PPP3R2. PPP3Rl is ubiquitously expressed in different tissues and is coded by the PPP3R1 gene which was mapped recently to human chromosome 2. PPP3Rl has four functional calcium-binding sites.

Publications

Species	Application	Title
human	IF	Autophagy; SMURF1 controls the PPP3/calcineurin complex and TFEB at a regulatory node for lysosomal biogenesis; Authors - Qin Xia
human	WB	Acta Biomater; Substrate stiffness regulates differentiation of induced pluripotent stem cells into heart valve endothelial cells.; Authors - Hong Cao
mouse	WB	Proc Natl Acad Sci U S A; Major contribution of the 3/6/7 class of TRPC channels to myocardial ischemia/reperfusion and cellular hypoxia/reoxygenation injuries.; Authors - Xiju He
human	WB	Biochem Biophys Res Commun; The calcineurin B subunit induces TNF-related apoptosis-inducing ligand (TRAIL) expression via CD11b-NF-κB pathway in RAW264.7 macrophages.; Authors - Su Zhenyi Z
chicken	WB	Biol Trace Elem Res; Transcriptional Study Revealed That Boron Supplementation May Alter the Immune-Related Genes Through MAPK Signaling in Ostrich Chick Thymus.; Authors - Ke Xiao
mouse	WB	Autophagy; Narirutin activates TFEB (transcription factor EB) to protect against acetaminophen-induced liver injury by targeting PPP3/calcineurin; Authors - Zhiyuan Fang

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