Anticorps Monoclonal anti-PPP4R1

PPP4R1 Monoclonal Antibody for WB, IHC, IF-P, FC (Intra), Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF-P, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

3D12G12

N° de cat : 67392-1-PBS

Synonymes

Serine/threonine-protein phosphatase 4 regulatory subunit 1, PP4R1, PP4(Rmeg), MEG1, 3D12G12

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Galerie de données de validation

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  WB analysis using 67392-1-Ig (same clone as 67392-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67392-1-Ig (PPP4R1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67392-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 67392-1-Ig (same clone as 67392-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67392-1-Ig (PPP4R1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67392-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 67392-1-Ig (same clone as 67392-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67392-1-Ig (PPP4R1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67392-1-PBS in a different storage buffer formulation.

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  IF Staining of human liver cancer using 67392-1-Ig (same clone as 67392-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using PPP4R1 antibody (67392-1-Ig, Clone: 3D12G12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67392-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse liver using 67392-1-Ig (same clone as 67392-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using PPP4R1 antibody (67392-1-Ig, Clone: 3D12G12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67392-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 67392-1-Ig (same clone as 67392-1-PBS)

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human PPP4R1 (67392-1-Ig, Clone:3D12G12) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 67392-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67392-1-PBS cible PPP4R1 dans les applications de WB, IHC, IF-P, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: PPP4R1 Protéine recombinante Ag26750
• Nom complet: protein phosphatase 4, regulatory subunit 1
• Masse moléculaire calculée: 107 kDa
• Poids moléculaire observé: 120-130 kDa
• Numéro d’acquisition GenBank: BC060829
• Symbole du gène: PPP4R1
• Identification du gène (NCBI): 9989
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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