Anticorps Polyclonal de lapin anti-PPP4R2

PPP4R2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications(1)

N° de cat : 22676-1-AP

Synonymes

PP4R2, PPP4R2

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Galerie de données de validation

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  WB analysis of A549 using 22676-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 22676-1-AP (PPP4R2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 22676-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 22676-1-AP (PPP4R2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 22676-1-AP

  IP result of anti-PPP4R2 (IP:22676-1-AP, 4ug; Detection:22676-1-AP 1:1000) with HepG2 cells lysate 3600ug.

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  IHC staining of human liver cancer using 22676-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 22676-1-AP (PPP4R2 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver cancer using 22676-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 22676-1-AP (PPP4R2 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human pancreas using 22676-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 22676-1-AP (PPP4R2 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human pancreas using 22676-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 22676-1-AP (PPP4R2 Antibody) at dilution of 1:50 (under 25x lens).

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  IF Staining of HepG2 using 22676-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 22676-1-AP (PPP4R2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules HepG2
• Résultats positifs en IP: cellules HepG2
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu pancréatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

22676-1-AP cible PPP4R2 dans les applications de WB, IHC, IF/ICC, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PPP4R2 Protéine recombinante Ag18486
• Nom complet: protein phosphatase 4, regulatory subunit 2
• Masse moléculaire calculée: 360aa,40 kDa; 495aa,55 kDa
• Poids moléculaire observé: 65 kDa
• Numéro d’acquisition GenBank: BC110889
• Symbole du gène: PPP4R2
• Identification du gène (NCBI): 151987
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PPP4R2 is a regulatory subunit of protein phosphatase-4 (PPP4), a protein serine/threonine phosphatase implicated in microtubule organization at centrosomes and DNA double-strand break repair. The deduced 453-amino acid protein contains several potential phosphorylation sites and has a calculated molecular mass of 50.4 kD. Immunofluorescent analysis of HeLa and A431 cells revealed prominent localization of PPP4R2 at centrosomes, with weaker staining of cytoplasm and nucleus(PMID: 10769191).

Publications

Species	Application	Title
human	WB,CoIP	Exp Cell Res; Protein phosphatase 4 mediates palmitic acid-induced endothelial dysfunction by decreasing eNOS phosphorylation at serine 633 in HUVECs; Authors - Zhengwei Liang

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