Anticorps Polyclonal de lapin anti-PPPDE1/PNAS4

• Phare
• Validé par KD/KO

PPPDE1/PNAS4 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (2)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(7)

N° de cat : 20517-1-AP

Synonymes

PPPDE1, EC:3.1.2.22, Deubiquitinase DESI2, Desumoylating isopeptidase 2, DeSI-2

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Galerie de données de validation

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  WB analysis of A549 cells using 20517-1-AP

  WB result of PPPDE1 antibody (20517-1-AP, 1:1000) with A549 cells transfected with si-control and si-PPPDE1.

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  WB analysis using 20517-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20517-1-AP (PPPDE1/PNAS4 antibody) at dilution of 1:7000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 20517-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20517-1-AP (PPPDE1/PNAS4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 20517-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20517-1-AP (PPPDE1/PNAS4 antibody) at dilution of 1:7000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 20517-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 20517-1-AP (PPPDE1/PNAS4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of A549 using 20517-1-AP

  IP result of anti-PPPDE1/PNAS4 (IP:20517-1-AP, 4ug; Detection:20517-1-AP 1:500) with A549 cells lysate 2800ug.

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  IHC staining of human lung cancer using 20517-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 20517-1-AP (PPPDE1/PNAS4 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung cancer using 20517-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 20517-1-AP (PPPDE1/PNAS4 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 20517-1-AP

  Immunofluorescent analysis of HepG2 cells, using PPPDE1 antibody 20517-1-AP at 1:25 dilution and FITC- labeled goat anti-Rabbit IgG (green).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules A549, cellules NCI-H1299, cellules U-251
• Résultats positifs en IP: cellules A549
• Résultats positifs en IHC: tissu de cancer du poumon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:300-1:600
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 6 publications below
• IHC: See 5 publications below
• IF: See 2 publications below

Informations sur le produit

20517-1-AP cible PPPDE1/PNAS4 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PPPDE1/PNAS4 Protéine recombinante Ag14352
• Nom complet: PPPDE peptidase domain containing 1
• Masse moléculaire calculée: 194 aa, 21 kDa
• Poids moléculaire observé: 23 kDa
• Numéro d’acquisition GenBank: BC004485
• Symbole du gène: PPPDE1
• Identification du gène (NCBI): 51029
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PPPDE peptidase domain 1 (PPPDE1), also named as PNAS4, is a highly conserved gene ubiquitously expressed in various tissues. PPPDE1 has been identified as a novel pro-apoptotic gene activated during the early response to DNA damage which is thought to play a critical role in cellular function regarding the maintenance of genomic integrity. This antibody specifically recognized endogenous PPPDE1, which has been confirmed by siRNA test.

Publications

Species	Application	Title
human	WB	Apoptosis; PPPDE1 promotes hepatocellular carcinoma development by negatively regulate p53 and apoptosis.; Authors - Xingwang Xie
rat	IHC, IF, WB	Exp Cell Res; Upregulation of PPPDE1 contributes to anorectal malformations via the mitochondrial apoptosis pathway during hindgut development in rats.; Authors - Si Ying Li
human,mouse	WB,IHC,IF	Biochimie; Comprehensive analysis of expression profile reveals the ubiquitous distribution of PPPDE peptidase domain 1, a Golgi apparatus component, and its implications in clinical cancer.; Authors - He Yi Y
human	WB	Biochem Biophys Res Commun; PPPDE1 is a novel deubiquitinase belonging to a cysteine isopeptidase family.; Authors - Xingwang Xie
human	IHC,WB	Pathol Oncol Res; High Phosphorylation Status of AKT/mTOR Signal in DESI2-Reduced Pancreatic Ductal Adenocarcinoma.; Authors - Cong-Cong Shen
human	WB,IHC	Oncol Lett; Analysis of transcription profile to reveal altered signaling pathways following the overexpression of human desumoylating isopeptidase 2 in pancreatic cancer cells.; Authors - Yu-Yin Fu

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