Anticorps Recombinant de lapin anti-PQBP1

PQBP1 Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

241901B11

N° de cat : 84491-6-PBS

Synonymes

241901B11, Npw38, Polyglutamine tract-binding protein 1, Polyglutamine-binding protein 1, PQBP-1

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Galerie de données de validation

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  WB analysis using 84491-6-RR (same clone as 84491-6-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84491-6-RR (PQBP1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84491-6-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 84491-6-RR (same clone as 84491-6-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 84491-6-RR (PQBP1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84491-6-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 84491-6-RR (same clone as 84491-6-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 84491-6-RR (PQBP1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84491-6-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 84491-6-RR (same clone as 84491-6-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84491-6-RR (PQBP1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84491-6-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 84491-6-RR (same clone as 84491-6-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PQBP1 antibody (84491-6-RR, Clone: 241901B11 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84491-6-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 84491-6-RR (same clone as 84491-6-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PQBP1 antibody (84491-6-RR, Clone: 241901B11 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84491-6-PBS in a different storage buffer formulation.

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  IF Staining of NIH/3T3 using 84491-6-RR (same clone as 84491-6-PBS)

  Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using PQBP1 antibody (84491-6-RR, Clone: 241901B11 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84491-6-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84491-6-RR

  Biolayer interferometry (BLl) kinetic assays of 84491-6-RR against Human PQBP1 were performed. The affinity constant is 0.529 nM.

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Informations sur le produit

84491-6-PBS cible PQBP1 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: PQBP1 Protéine recombinante Ag9329
• Nom complet: polyglutamine binding protein 1
• Masse moléculaire calculée: 265 aa, 30 kDa
• Poids moléculaire observé: 30-32 kDa
• Numéro d’acquisition GenBank: BC012358
• Symbole du gène: PQBP1
• Identification du gène (NCBI): 10084
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

PQBP1 (Polyglutamine-binding protein 1) is also named as NPW38 and JM26. PQBP1 senses extrinsic tau 3R/4R proteins by direct interaction and triggers an innate immune response by activating a cyclic GMP-AMP synthase (cGAS)-Stimulator of interferon genes (STING) pathway (PMID: 34782623).  PQBP1 is abundantly expressed in the central nervous system during development. PQBP1 is a component of the precatalytic spliceosome (B complex) that is involved in splicing. PQBP1 was predominantly localized in the cell nucleus. PQBP1 is overexpressed in various cancer types including breast cancer, colon cancer, and glioblastoma (PMID: 38342602).

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