Anticorps Polyclonal de lapin anti-PRAS40

PRAS40 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

N° de cat : 32456-1-AP

Synonymes

AKT1S1, 40 kDa proline rich AKT substrate, 40 kDa proline-rich AKT substrate, Proline rich AKT1 substrate 1, Proline-rich AKT1 substrate 1

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Galerie de données de validation

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  WB analysis using 32456-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 32456-1-AP (PRAS40 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis using 32456-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 32456-1-AP (PRAS40 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 32456-1-AP

  IP result of anti-PRAS40 (IP:32456-1-AP, 4ug; Detection:32456-1-AP 1:2000) with HeLa cells lysate 1480 ug.

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  IF Staining of HeLa using 32456-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PRAS40 antibody (32456-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules HeLa, cellules HepG2, cellules L02
• Résultats positifs en IP: cellules HeLa,
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

32456-1-AP cible PRAS40 dans les applications de WB, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PRAS40 Protéine recombinante Ag35446
• Nom complet: AKT1 substrate 1 (proline-rich)
• Masse moléculaire calculée: 27 kDa
• Poids moléculaire observé: 40 kDa
• Numéro d’acquisition GenBank: BC051844
• Symbole du gène: PRAS40
• Identification du gène (NCBI): 84335
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PRAS40 (40 kDa proline-rich AKT substrate) is also named as AKT1S1 (Proline-rich AKT1 substrate 1). PRAS40 is a substrate of Akt and a component of the mammalian target of rapamycin complex 1 (mTORC1) (PMID: 28978182). Locating at the crossroad of the PI3K/Akt pathway and the mTOR pathway, PRAS40 is phosphorylated by growth factors or other stimuli, and regulates the activation of these signaling pathways in turn (PMID: 28978182). PRAS40 is expressed in a variety of tissues in vivo and has multiple phosphorylation sites, which its activity is closely related to phosphorylation. Studies have shown that PRAS40 is involved in regulating cell growth, cell apoptosis, oxidative stress, autophagy and angiogenesis, as well as various of signalling pathways such as mammalian target of mammalian target rapamycin (mTOR), protein kinase B (PKB/Akt), nuclear factor kappa-B(NF-κB) proto-oncogene serine/threonine-protein kinase PIM-1(PIM1) and pyruvate kinase M2 (PKM2) (PMID: 33504218).

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