Anticorps Monoclonal anti-PRC1

• Phare
• Validé par KD/KO

PRC1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1E1D5

N° de cat : 67027-1-PBS

Synonymes

1E1D5, ASE1

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Galerie de données de validation

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  WB analysis using 67027-1-Ig (same clone as 67027-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67027-1-Ig (PRC1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67027-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 67027-1-Ig (same clone as 67027-1-PBS)

  WB result of PRC1 antibody (67027-1-Ig; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PRC1 transfected MCF-7 cells. This data was developed using the same antibody clone with 67027-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67027-1-Ig (same clone as 67027-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67027-1-Ig (PRC1 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67027-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67027-1-Ig (same clone as 67027-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67027-1-Ig (PRC1 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67027-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 67027-1-Ig (same clone as 67027-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67027-1-Ig (PRC1 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67027-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 67027-1-Ig (same clone as 67027-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67027-1-Ig (PRC1 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67027-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 67027-1-Ig (same clone as 67027-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PRC1 antibody (67027-1-Ig, Clone: 1E1D5 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67027-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 67027-1-Ig (same clone as 67027-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PRC1 antibody (67027-1-Ig, Clone: 1E1D5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67027-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67027-1-PBS cible PRC1 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: PRC1 Protéine recombinante Ag5907
• Nom complet: protein regulator of cytokinesis 1
• Masse moléculaire calculée: 72 kDa
• Poids moléculaire observé: 66-72 kDa
• Numéro d’acquisition GenBank: BC003138
• Symbole du gène: PRC1
• Identification du gène (NCBI): 9055
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

PRC1 (protein regulator of cytokinesis 1) is a microtubule-bundling protein that is involved in cytokinesis. Depletion of PRC1 leads to failed cytokinesis. PRC1 is located in the nucleus during interphase, and becomes associated with mitotic spindles in a highly dynamic manner during mitosis, and localizes to the cell mid-body during cytokinesis. PRC1 is a substrate of CDK1 and it was assumed that CDK1 phosphorylation blocks its activity during metaphase. Three isoforms of PRC1 exist due to the alternative splicing, with predictive molecular weight of 71.6 kDa, 70.2 kDa and 66.2 kDa, respectively. The dual bands between 66-72 kDa detected by this antibody may represent different forms of PRC1.

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