Anticorps Recombinant de lapin anti-PRC1

PRC1 Recombinant Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

241216H2

N° de cat : 84041-2-PBS

Synonymes

241216H2, ASE1

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Galerie de données de validation

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  WB analysis using 84041-2-RR (same clone as 84041-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84041-2-RR (PRC1 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84041-2-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 84041-2-RR (same clone as 84041-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PRC1 antibody (84041-2-RR, Clone: 241216H2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84041-2-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 84041-2-RR (same clone as 84041-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PRC1 antibody (84041-2-RR, Clone: 241216H2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red). This data was developed using the same antibody clone with 84041-2-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 84041-2-RR (same clone as 84041-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PRC1 antibody (84041-2-RR, Clone: 241216H2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red). This data was developed using the same antibody clone with 84041-2-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 84041-2-RR (same clone as 84041-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PRC1 antibody (84041-2-RR, Clone: 241216H2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red). This data was developed using the same antibody clone with 84041-2-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 84041-2-RR (same clone as 84041-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PRC1 antibody (84041-2-RR, Clone: 241216H2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red). This data was developed using the same antibody clone with 84041-2-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 84041-2-RR (same clone as 84041-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PRC1 antibody (84041-2-RR, Clone: 241216H2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red). This data was developed using the same antibody clone with 84041-2-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00970-1

  Cytometric bead array standard curve of MP00970-1, PRC1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84041-2-PBS. Detection antibody: 84041-1-PBS. Standard: Ag8028. Range: 0.625-80 ng/mL.

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  Cytometric bead array standard curve of MP00970-2

  Cytometric bead array standard curve of MP00970-2, PRC1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84041-2-PBS. Detection antibody: 84041-3-PBS. Standard:null. Range: null.

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  Cytometric bead array standard curve of MP00970-2

  Cytometric bead array standard curve of MP00970-2, PRC1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84041-2-PBS. Detection antibody: 84041-3-PBS. Standard: Ag8028. Range: 0.625-80 ng/mL.

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  Affinity and Kinetic Characterization of 84041-2-RR

  Biolayer interferometry (BLl) kinetic assays of 84041-2-RR against Human PRC1 were performed. The affinity constant is below 1 pM.

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Informations sur le produit

84041-2-PBS cible PRC1 dans les applications de WB, IF/ICC, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: PRC1 Protéine recombinante Ag8028
• Nom complet: protein regulator of cytokinesis 1
• Masse moléculaire calculée: 72 kDa
• Poids moléculaire observé: 66-72 kDa
• Numéro d’acquisition GenBank: BC003138
• Symbole du gène: PRC1
• Identification du gène (NCBI): 9055
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

PRC1 (protein regulator of cytokinesis 1) is a microtubule-bundling protein that is involved in cytokinesis. Depletion of PRC1 leads to failed cytokinesis. PRC1 is located in the nucleus during interphase, and becomes associated with mitotic spindles in a highly dynamic manner during mitosis, and localizes to the cell mid-body during cytokinesis. PRC1 is a substrate of CDK1 and it was assumed that CDK1 phosphorylation blocks its activity during metaphase. Three isoforms of PRC1 exist due to the alternative splicing, with predictive molecular weight of 71.6 kDa, 70.2 kDa and 66.2 kDa, respectively.

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