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Anticorps Polyclonal de lapin anti-PRDM15

PRDM15 Polyclonal Antibody for WB, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 25590-1-PBS

Synonymes

Galerie de données de validation

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  • Western Blot (WB) analysis of Jurkat cells using PRDM15 Polyclonal antibody (25590-1-AP)

    WB analysis of Jurkat cells using 25590-1-AP (same clone as 25590-1-PBS)

    WB result of PRDM15 (25590-1-AP, 1:500) with si-control and si-PRDM15 transfected Jurkat cells. This data was developed using the same antibody clone with 25590-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using PRDM15 Polyclonal antibody (25590-1-AP)

    WB analysis of MCF-7 using 25590-1-AP (same clone as 25590-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 25590-1-AP (PRDM15 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25590-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of MCF-7 cells using PRDM15 Polyclonal antibody (25590-1-AP)

    IP experiment of MCF-7 using 25590-1-AP (same clone as 25590-1-PBS)

    IP result of anti-PRDM15 (IP:25590-1-AP, 5ug; Detection:25590-1-AP 1:500) with MCF-7 cells lysate 2500ug. This data was developed using the same antibody clone with 25590-1-PBS in a different storage buffer formulation.

Informations sur le produit

25590-1-PBS cible PRDM15 dans les applications de WB, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain

Réactivité Humain
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène PRDM15 Protéine recombinante Ag22296
Nom complet PR domain containing 15
Masse moléculaire calculée 1507 aa, 169 kDa
Poids moléculaire observé 169 kDa
Numéro d’acquisition GenBankBC067102
Symbole du gène PRDM15
Identification du gène (NCBI) 63977
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par affinité contre l'antigène
Tampon de stockage PBS only
Conditions de stockageStore at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

The PR-domain containing proteins (PRDMs) have a common involvement in the modulation of gene activities. A PR-domain family member usually produces two products, called PR-plus and PR-minus, which differ by the presence or absence of the PR domain, respectively. The PR-plus product is underexpressed or disrupted in cancer cells, whereas the PR-minus product is present or overexpressed in cancer cells. This imbalance in the amount of the two products, which is a result of either genetic or epigenetic events, appears to be a determining factor of malignancy. PRDM15 (PR domain-containing protein 15), also known as PFM15 or ZNF298, is a 1507 amino acid protein belonging to the PRDM family. Localizing to the nucleus, PRDM15 contains sixteen C2H2-type zinc fingers and one SET domain. It is believed to participate in transcriptional regulation and may be involved in cell differentiation and tumorigenesis. PRDM15 exists several isoform and molecular weight of respective isoform is 169 kDa, 135kda, 62kDa.

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