Anticorps Recombinant de lapin anti-PRDM16

PRDM16 Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

240662D3

N° de cat : 83872-1-PBS

Synonymes

MDS1/EVI1-like gene 1, MDS1/EVI1 like gene 1, KIAA1675, Histone-lysine N-methyltransferase PRDM16, EC:2.1.1.367

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Galerie de données de validation

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  WB analysis using 83872-1-RR (same clone as 83872-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83872-1-RR (PRDM16 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83872-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brown adipose using 83872-1-RR (same clone as 83872-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brown adipose tissue slide using 83872-1-RR (PRDM16 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83872-1-PBS in a different storage buffer formulation.

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  IF Staining of HEK-293 using 83872-1-RR (same clone as 83872-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using PRDM16 antibody (83872-1-RR, Clone: 240662D3 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83872-1-PBS in a different storage buffer formulation.

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Informations sur le produit

83872-1-PBS cible PRDM16 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: PR domain containing 16
• Masse moléculaire calculée: 140 kDa
• Poids moléculaire observé: ~140 kDa
• Numéro d’acquisition GenBank: NM_022114
• Symbole du gène: PRDM16
• Identification du gène (NCBI): 63976
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

PR domain containing 16, also known as PRDM16, acts as a transcription coregulator that controls the development of brown adipocytes in brown adipose tissue. Previously, this coregulator was believed to be present only in brown adipose tissue, but more recent studies have shown that PRDM16 is highly expressed in subcutaneous white adipose tissue as well. The protein encoded by this gene is a zinc finger transcription factor. PRDM16 controls the cell fate between muscle and brown fat cells. Loss of PRDM16 from brown fat precursors causes a loss of brown fat characteristics and promotes muscle differentiation.

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