Anticorps Recombinant de lapin anti-PRDM8

PRDM8 Recombinant Antibody for WB, IHC, IF-P, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF-P, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

242203D8

N° de cat : 84716-2-PBS

Synonymes

242203D8, EC:2.1.1.-, PR domain containing 8, PR domain containing protein 8, PR domain-containing protein 8

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Galerie de données de validation

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  WB analysis using 84716-2-RR (same clone as 84716-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84716-2-RR (PRDM8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84716-2-PBS in a different storage buffer formulation.

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  IHC staining of mouse eye using 84716-2-RR (same clone as 84716-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 84716-2-RR (PRDM8 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84716-2-PBS in a different storage buffer formulation.

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  IHC staining of mouse eye using 84716-2-RR (same clone as 84716-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 84716-2-RR (PRDM8 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84716-2-PBS in a different storage buffer formulation.

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  IF Staining of mouse eye using 84716-2-RR (same clone as 84716-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse eye tissue using PRDM8 antibody (84716-2-RR, Clone: 242203D8 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84716-2-PBS in a different storage buffer formulation.

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  IF Staining of mouse eye using 84716-2-RR (same clone as 84716-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse eye tissue using PRDM8 antibody (84716-2-RR, Clone: 242203D8 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84716-2-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP01532-1

  Cytometric bead array standard curve of MP01532-1, PRDM8 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84716-2-PBS. Detection antibody: 84716-3-PBS. Standard: Ag23373. Range: 0.781-100 ng/mL.

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  Affinity and Kinetic Characterization of 84716-2-RR

  Biolayer interferometry (BLl) kinetic assays of 84716-2-RR against Human PRDM8 were performed. The affinity constant is 31.8 nM.

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Informations sur le produit

84716-2-PBS cible PRDM8 dans les applications de WB, IHC, IF-P, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: PRDM8 Protéine recombinante Ag23373
• Nom complet: PR domain containing 8
• Masse moléculaire calculée: 689 aa, 72 kDa
• Poids moléculaire observé: 72 kDa
• Numéro d’acquisition GenBank: BC071584
• Symbole du gène: PRDM8
• Identification du gène (NCBI): 56978
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

PRDI-BF1 and RIZ homology domain zinc fnger protein 8 (PRDM8) belongs to the PRDM family characterized by a conserved N-terminal PR domain related to the catalytic SET domains. PRDM8 is a key regulator in neural development and testis steroidogenesis (PMID: 32819411). PRDM8 is mainly demonstrated as a crucial regulatory molecule during neural development and testis steroidogen and expressed at low levels in HCC tissues (PMID: 29572888).

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