Anticorps Monoclonal anti-PRDX1

• Phare
• Validé par KD/KO

PRDX1 Monoclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugaison

Non conjugué

Publications

3

CloneNo.

2B2A2

Avis (1)

N° de cat : 66820-1-Ig

Synonymes

MSP23, NKEF A, NKEFA, PAG, PAGA, PAGB, peroxiredoxin 1, PRDX1, PRX1, PRXI, TDPX2, Thioredoxin peroxidase 2

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Galerie de données de validation

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  WB analysis using 66820-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66820-1-Ig (PRDX1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 66820-1-Ig

  WB result of PRDX1 antibody (66820-1-Ig; 1:200000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PRDX1 transfected HEK-293 cells.

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  WB analysis of U2OS using 66820-1-Ig

  U2OS (WT and PRDX1 KO) lysates prepared with RIPA buffer, 10 μg protein loaded. 66820-1-Ig incubated at 1:5000 at 4°C overnight in 5% BSA in TBST. Ponceau stained transfers shown on right. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.

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  WB analysis using 66820-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66820-1-Ig (PRDX1 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 66820-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66820-1-Ig (PRDX1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 66820-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66820-1-Ig (PRDX1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human liver cancer using 66820-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using PRDX1 antibody (66820-1-Ig, Clone: 2B2A2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules 4T1, cellules Caco-2, cellules HEK-293, cellules HSC-T6, cellules Jurkat, cellules LNCaP, cellules MCF-7, cellules NIH/3T3, cellules U2OS
• Résultats positifs en IHC: tissu de cancer du foie humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu de cancer du foie humain,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

66820-1-Ig cible PRDX1 dans les applications de WB, IP, IHC, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: PRDX1 Protéine recombinante Ag8821
• Nom complet: peroxiredoxin 1
• Masse moléculaire calculée: 199 aa, 22 kDa
• Poids moléculaire observé: 23 kDa
• Numéro d’acquisition GenBank: BC007063
• Symbole du gène: PRDX1
• Identification du gène (NCBI): 5052
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PRDX1(Peroxiredoxin-1), also named as PAGA, PAGB, TDPX2, PAG or NKEF-A, belongs to the ahpC/TSA family. PRDX1 is a thiol reductase that plays critical roles in oxidative and thermal stress defense mechanisms through its abilities to metabolize H2O2 and act as a molecular chaperone, respectively. PRDX1 might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H2O2 ((PMID: 9497357)). It reduces an intramolecular disulfide bond in GDPD5 that gates the ability to GDPD5 to drive postmitotic motor neuron differentiation. PRDX1 can form a dimer, and also can be phosphorylated on Thr-90 during the M-phase, which leads to a more than 80% decrease in enzymatic activity (PMID: 22583657, 11986303).

Publications

Species	Application	Title
human	WB, CoIP	J Virol; Peroxiredoxin 1 interacts with TBK1/IKKε and negatively regulates pseudorabies virus propagation by promoting innate immunity.; Authors - Lin Lv
mouse	WB	Antioxidants (Basel); Garlic-Derived Metabolites Exert Antioxidant Activity, Modulate Gut Microbiota Composition and Limit Citrobacter rodentium Infection in Mice; Authors - Ling Zhu
rat	IP	Heliyon; EEF1A2 accelerates the protein translation of chemokine in rat myocardial cells induced by ischemia-reperfusion; Authors - Zi-Jie Zhang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

PILAR (Verified Customer) (08-24-2023)

Excellent customer service and fast shipment. Waiting for the experiment results!

• Applications: Western Blot