Anticorps Polyclonal de lapin anti-peroxiredoxin 2

• Phare
• Validé par KD/KO

peroxiredoxin 2 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, RIP, IP, IHC, IF, CoIP, ELISA

Conjugaison

Non conjugué

Publications

45

N° de cat : 10545-2-AP

Synonymes

NKEF B, NKEFB, peroxiredoxin 2, PRDX2, PRP, PRX II, PRX2, PRXII, TDPX1, Thioredoxin peroxidase 1, TSA

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Galerie de données de validation

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  WB analysis using 10545-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10545-2-AP (peroxiredoxin 2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 10545-2-AP

  WB result of peroxiredoxin 2 antibody (10545-2-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-peroxiredoxin 2 transfected HepG2 cells.

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  IP experiment of HepG2 using 10545-2-AP

  IP result of anti-peroxiredoxin 2(IP:10545-2-AP, 4ug; Detection:10545-2-AP 1:4000) with HepG2 cells lysate 960 ug.

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  IHC staining of human liver using 10545-2-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 10545-2-AP (peroxiredoxin 2 antibody) at dilution of 1:600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 10545-2-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 10545-2-AP (peroxiredoxin 2 antibody) at dilution of 1:600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human hepatocirrhosis using 10545-2-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 10545-2-AP (peroxiredoxin 2 antibody) at dilution of 1:600 (under 10x lens).

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  IHC staining of human hepatocirrhosis using 10545-2-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 10545-2-AP (peroxiredoxin 2 antibody) at dilution of 1:600 (under 40x lens).

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  IF Staining of HepG2 using 10545-2-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using peroxiredoxin 2 antibody (10545-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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  IF Staining of HeLa using 10545-2-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using peroxiredoxin 2 antibody (10545-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules C2C12, cellules PC-12
• Résultats positifs en IP: cellules HepG2,
• Résultats positifs en IHC: tissu hépatique humain, tissu de cirrhose hépatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2, cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:600-1:3000
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 5 publications below
• WB: See 38 publications below
• IHC: See 11 publications below
• IF: See 4 publications below
• IP: See 3 publications below
• CoIP: See 1 publications below
• RIP: See 1 publications below

Informations sur le produit

10545-2-AP cible peroxiredoxin 2 dans les applications de WB, RIP, IP, IHC, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: peroxiredoxin 2 Protéine recombinante Ag0835
• Nom complet: peroxiredoxin 2
• Masse moléculaire calculée: 22 kDa
• Poids moléculaire observé: 22 kDa
• Numéro d’acquisition GenBank: BC003022
• Symbole du gène: PRDX2
• Identification du gène (NCBI): 7001
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PRDX2, also named as TSA, PRP, NKEFB and TDPX1, belongs to the ahpC/TSA family. It is known to act as an antioxidant enzyme whose main function is H2O2 reduction in cells. PRDX2 is involved in redox regulation of the cell. It reduces peroxides with reducing equivalents provided through the thioredoxin system. It may play an important role in eliminating peroxides generated during metabolism. PRDX2 might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H2O2. PRDX2 actions may be related to the expression of NFKB and IKB.(PMID:21248284)

Publications

Species	Application	Title
human	WB	ACS Nano; Engineering Extracellular Vesicles Restore the Impaired Cellular Uptake and Attenuate Intervertebral Disc Degeneration.; Authors - Zhiwei Liao
rat	WB	Br J Pharmacol; Luteolin ameliorates rat myocardial ischemia-reperfusion injury through peroxiredoxin II activation.; Authors - Bo Wei
human	WB, IHC	Oncogene; PMID: 26500057; Authors - Y-H Park
human,mouse	WB,IP,CoIP	Free Radic Biol Med; Oxidation resistance 1 regulates post-translational modifications of peroxiredoxin 2 in the cerebellum.; Authors - Daria M Svistunova
mouse	IF	Stem Cells; Dominant role of peroxiredoxin/JNK axis in stemness regulation during neurogenesis from embryonic stem cells.; Authors - Sun-Uk Kim
human	WB,IHC	Oxid Med Cell Longev; Azelaic Acid Exerts Antileukemia Effects against Acute Myeloid Leukemia by Regulating the Prdxs/ROS Signaling Pathway.; Authors - Dongdong Zhang