Anticorps Monoclonal anti-peroxiredoxin 2

• Phare
• Validé par KD/KO

peroxiredoxin 2 Monoclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

5

CloneNo.

3H6C4

N° de cat : 60202-1-Ig

Synonymes

NKEF B, NKEFB, peroxiredoxin 2, PRDX2, PRP, PRX2, PRXII, TDPX1, Thioredoxin peroxidase 1, TSA

---

Galerie de données de validation

• 

  WB analysis using 60202-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60202-1-Ig (peroxiredoxin 2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 60202-1-Ig

  WB result of peroxiredoxin 2 antibody (60202-1-Ig; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-peroxiredoxin 2 transfected HepG2 cells.

• 

  WB analysis of MCF-7 using 60202-1-Ig

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 60202-1-Ig (peroxiredoxin 2 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 60202-1-Ig

  human testis tissue were subjected to SDS PAGE followed by western blot with 60202-1-Ig (peroxiredoxin 2 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of fetal human brain using 60202-1-Ig

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 60202-1-Ig (peroxiredoxin 2 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 60202-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 60202-1-Ig (peroxiredoxin 2 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human testis using 60202-1-Ig

  Immunohistochemical analysis of paraffin-embedded human testis using 60202-1-Ig(peroxiredoxin 2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 60202-1-Ig

  Immunohistochemical analysis of paraffin-embedded human testis using 60202-1-Ig(peroxiredoxin 2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 60202-1-Ig

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 60202-1-Ig (peroxiredoxin 2 antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 60202-1-Ig

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 60202-1-Ig (peroxiredoxin 2 antibody at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human brain using 60202-1-Ig

  Immunohistochemical analysis of paraffin-embedded human brain using 60202-1-Ig(peroxiredoxin 2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 60202-1-Ig

  Immunohistochemical analysis of paraffin-embedded human brain using 60202-1-Ig(peroxiredoxin 2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of MCF-7 using 60202-1-Ig

  Immunofluorescent analysis of (-20℃ Ethanol ) fixed MCF-7 cells using 60202-1-Ig(peroxiredoxin 2 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

---

Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK-293, cellules HepG2, cellules HSC-T6, cellules Jurkat, cellules K-562, cellules MCF-7, cellules NIH/3T3, cellules PC-12, tissu cérébral humain fœtal, tissu testiculaire humain
• Résultats positifs en IHC: tissu testiculaire humain, tissu cérébral humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules MCF-7

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Informations sur le produit

60202-1-Ig cible peroxiredoxin 2 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: peroxiredoxin 2 Protéine recombinante Ag0835
• Nom complet: peroxiredoxin 2
• Masse moléculaire calculée: 22 kDa
• Poids moléculaire observé: 22 kDa
• Numéro d’acquisition GenBank: BC003022
• Symbole du gène: PRDX2
• Identification du gène (NCBI): 7001
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PRDX2, also named as TSA, PRP, NKEFB and TDPX1, belongs to the ahpC/TSA family. It is known to act as an antioxidant enzyme whose main function is H2O2 reduction in cells. PRDX2 is involved in redox regulation of the cell. It reduces peroxides with reducing equivalents provided through the thioredoxin system. It may play an important role in eliminating peroxides generated during metabolism. PRDX2 might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H2O2. PRDX2 actions may be related to the expression of NFKB and IKB.(PMID:21248284)

Publications

Species	Application	Title
human	WB,IHC,IF	J Exp Clin Cancer Res; Compound kushen injection suppresses human acute myeloid leukaemia by regulating the Prdxs/ROS/Trx1 signalling pathway.; Authors - Yanxia Jin
human	WB	Cancer Manag Res; siPRDX2-elevated DNM3 inhibits the proliferation and metastasis of colon cancer cells via AKT signaling pathway.; Authors - Yini Ma ; KD Validated
human	WB	Oncol Lett; PRDX2 protects hepatocellular carcinoma SMMC-7721 cells from oxidative stress.; Authors - Silei Zhou
human	IF	Cell Death Dis; PRDX2 promotes the proliferation of colorectal cancer cells by increasing the ubiquitinated degradation of p53.; Authors - Wuyi Wang ; KD Validated
human	WB	Curr Issues Mol Biol; Peroxiredoxins and Hypoxia-Inducible Factor-1α in Duodenal Tissue: Emerging Factors in the Pathophysiology of Pediatric Celiac Disease Patients; Authors - Fadime Aydın Köse