Anticorps Polyclonal de lapin anti-PRDX3

PRDX3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(6)

N° de cat : 55087-1-AP

Synonymes

peroxiredoxin 3, MER5, EC:1.11.1.24, AOP-1, AOP1

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Galerie de données de validation

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  WB analysis using 55087-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55087-1-AP (PRDX3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 55087-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55087-1-AP (PRDX3 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 55087-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 55087-1-AP (PRDX3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 55087-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 55087-1-AP (PRDX3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A549 using 55087-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using PRDX3 antibody (55087-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HepG2, cellules MCF-7, cellules THP-1, tissu hépatique de rat, tissu hépatique de souris
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu de cancer de l'estomac humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules A549,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below
• IF: See 1 publications below

Informations sur le produit

55087-1-AP cible PRDX3 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: peroxiredoxin 3
• Masse moléculaire calculée: 28 kDa
• Poids moléculaire observé: 26-28 kDa
• Numéro d’acquisition GenBank: NM_006793
• Symbole du gène: PRDX3
• Identification du gène (NCBI): 10935
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PRDX3(Peroxiredoxin 3), also named as AOP1, HBC189 and MER5, belongs to the ahpC/TSA family. It is involved in redox regulation of the cell and protects radical-sensitive enzymes from oxidative damage by a radical-generating system. PRDX3 is required for MYC-mediated proliferation, transformation, and apoptosis after glucose withdrawal and essential for maintaining mitochondrial mass and membrane potential in transformed rat and human cells(PMID:12011429). PRDX3 acts synergistically with MAP3K13 to regulate the activation of NF-kappa-B in the cytosol. This antibody is specific to PRDX3.

Publications

Species	Application	Title
human		Science; Golgi-derived PI(4)P-containing vesicles drive late steps of mitochondrial division.; Authors - Shun Nagashima
rat	WB	Br J Pharmacol; Luteolin ameliorates rat myocardial ischemia-reperfusion injury through peroxiredoxin II activation.; Authors - Bo Wei
	WB	J Endocrinol; NNT is a key regulator of adrenal redox homeostasis and steroidogenesis in male mice.; Authors - Eirini Meimaridou
human	WB	J Cell Biochem; Molecular basis underlying the biological effects elicited by extremely low-frequency magnetic field (ELF-MF) on neuroblastoma cells.; Authors - Sulpizio Marilisa M
rat	WB	Cardiovasc Toxicol; Nrf1 Knock-Down in the Hypothalamic Paraventricular Nucleus Alleviates Hypertension Through Intervention of Superoxide Production-Removal Balance and Mitochondrial Function.; Authors - Ying Li
mouse	WB,IF	Respir Res; Peroxiredoxin 3 has a crucial role in the macrophage polarization by regulating mitochondrial homeostasis; Authors - Wenhui Huang

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