Anticorps Monoclonal anti-PRDX3

• Phare
• Validé par KD/KO

PRDX3 Monoclonal Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1F7F7

N° de cat : 66810-1-PBS

Synonymes

1F7F7, Antioxidant protein 1, AOP1, AOP-1, EC:1.11.1.24

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Galerie de données de validation

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  WB analysis using 66810-1-Ig (same clone as 66810-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66810-1-Ig (PRX3 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66810-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 66810-1-Ig (same clone as 66810-1-PBS)

  WB result of PRDX3 antibody (66810-1-Ig; 1:80000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PRDX3 transfected HEK-293 cells. This data was developed using the same antibody clone with 66810-1-PBS in a different storage buffer formulation.

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  WB analysis using 66810-1-Ig (same clone as 66810-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66810-1-Ig (PRDX3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66810-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66810-1-Ig (same clone as 66810-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66810-1-Ig (PRX3 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66810-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66810-1-Ig (same clone as 66810-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66810-1-Ig (PRX3 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66810-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66810-1-Ig (same clone as 66810-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PRDX3 antibody (66810-1-Ig, Clone: 1F7F7 ) at dilution of 1:1700 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 66810-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP50218-2

  Cytometric bead array standard curve of MP50218-2, PRDX3 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66810-1-PBS. Detection antibody: 66810-2-PBS. Standard: Ag1062. Range: 0.781-100 ng/mL.

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  Affinity and Kinetic Characterization of 66810-1-Ig

  Biolayer interferometry (BLl) kinetic assays of 66810-1-Ig against Human PRDX3 were performed. The affinity constant is 1.39 nM.

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Informations sur le produit

66810-1-PBS cible PRDX3 dans les applications de WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: PRDX3 Protéine recombinante Ag1062
• Nom complet: peroxiredoxin 3
• Masse moléculaire calculée: 27 kDa
• Poids moléculaire observé: 28 kDa
• Numéro d’acquisition GenBank: BC007062
• Symbole du gène: PRDX3
• Identification du gène (NCBI): 10935
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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