Anticorps Recombinant de lapin anti-PRDX3

• Phare
• Validé par KD/KO

PRDX3 Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1L11

N° de cat : 81833-1-PBS

Synonymes

1L11, Antioxidant protein 1, AOP1, AOP-1, EC:1.11.1.24

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Galerie de données de validation

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  WB analysis using 81833-1-RR (same clone as 81833-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 81833-1-RR (PRX3 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81833-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 81833-1-RR (same clone as 81833-1-PBS)

  WB result of PRDX3 antibody (81833-1-RR; 1:100000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PRDX3 transfected HEK-293 cells. This data was developed using the same antibody clone with 81833-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 81833-1-RR (same clone as 81833-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 81833-1-RR (PRDX3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81833-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 81833-1-RR (same clone as 81833-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 81833-1-RR (PRDX3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81833-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 81833-1-RR (same clone as 81833-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PRX3 antibody (81833-1-RR, Clone: 1L11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 81833-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 81833-1-RR (same clone as 81833-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PRDX3 antibody (81833-1-RR, Clone: 1L11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 81833-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 81833-1-PBS

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PRDX3 antibody (81833-1-PBS, Clone: 1L11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  Affinity and Kinetic Characterization of 81833-1-RR

  Biolayer interferometry (BLl) kinetic assays of 81833-1-RR against Human PRDX3 were performed. The affinity constant is below 1 pM.

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Applications testées

• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

81833-1-PBS cible PRDX3 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: PRDX3 Protéine recombinante Ag1062
• Nom complet: peroxiredoxin 3
• Masse moléculaire calculée: 27 kDa
• Poids moléculaire observé: 28 kDa
• Numéro d’acquisition GenBank: BC007062
• Symbole du gène: PRDX3
• Identification du gène (NCBI): 10935
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

PRDX3(Peroxiredoxin 3), also named as AOP1, HBC189 and MER5, belongs to the ahpC/TSA family. It is involved in redox regulation of the cell and protects radical-sensitive enzymes from oxidative damage by a radical-generating system. PRDX3 is required for MYC-mediated proliferation, transformation, and apoptosis after glucose withdrawal and essential for maintaining mitochondrial mass and membrane potential in transformed rat and human cells (PMID:12011429). PRDX3 acts synergistically with MAP3K13 to regulate the activation of NF-kappa-B in the cytosol.

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