Anticorps Monoclonal anti-PRDX4

• Phare
• Validé par KD/KO

PRDX4 Monoclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

2

CloneNo.

5B6F1

N° de cat : 60286-1-Ig

Synonymes

Antioxidant enzyme AOE372, AOE37 2, peroxiredoxin 4, Peroxiredoxin IV, PRDX4, PRX 4, Prx IV, Thioredoxin peroxidase AO372

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Galerie de données de validation

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  WB analysis of HepG2 using 60286-1-Ig

  WB result of PRDX4 antibody (60286-1-Ig, 1:5000) with si-Control and si-PRDX4 transfected HepG2 cells.

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  WB analysis using 60286-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60286-1-Ig (PRDX4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 60286-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 60286-1-Ig (PRDX4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-12 using 60286-1-Ig

  PC-12 cells were subjected to SDS PAGE followed by western blot with 60286-1-Ig (PRDX4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 60286-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 60286-1-Ig (PRDX4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 60286-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 60286-1-Ig (PRDX4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 60286-1-Ig

  K-562 cells were subjected to SDS PAGE followed by western blot with 60286-1-Ig (PRDX4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon using 60286-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon slide using 60286-1-Ig (PRDX4 Antibody) at dilution of 1:50(unde10x lens).

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  IHC staining of human colon using 60286-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon slide using 60286-1-Ig (PRDX4 Antibody) at dilution of 1:50(under 40x lens).

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  IHC staining of human pancreas cancer using 60286-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 60286-1-Ig (PRDX4 Antibody) at dilution of 1:50(under 10x lens).

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  IHC staining of human pancreas cancer using 60286-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 60286-1-Ig (PRDX4 Antibody) at dilution of 1:50(under 40x lens).

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  IHC staining of human prostate cancer using 60286-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 60286-1-Ig (PRDX4 Antibody) at dilution of 1:50(under 10x lens).

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  IHC staining of human prostate cancer using 60286-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 60286-1-Ig (PRDX4 Antibody) at dilution of 1:50(under 40x lens).

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  IF Staining of HepG2 using 60286-1-Ig

  Immunofluorescent analysis of HepG2 cells using 60286-1-Ig (PRDX4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).

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Applications testées

• Résultats positifs en WB: cellules LNCaP, cellules HEK-293, cellules HeLa, cellules HepG2, cellules HSC-T6, cellules Jurkat, cellules K-562, cellules NIH/3T3, cellules PC-12
• Résultats positifs en IHC: tissu de côlon humain, tissu de cancer de la prostate humain, tissu de cancer du pancréas humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

60286-1-Ig cible PRDX4 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: PRDX4 Protéine recombinante Ag13401
• Nom complet: peroxiredoxin 4
• Masse moléculaire calculée: 31 kDa
• Poids moléculaire observé: 30 kDa
• Numéro d’acquisition GenBank: BC007107
• Symbole du gène: PRDX4
• Identification du gène (NCBI): 10549
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PRDX4(Peroxiredoxin-4) is also named as AOE37-2, Prx-IV and belongs to the AhpC/TSA family. PRDX4 is associated with acrosome formation during rat spermatogenesis and has a protective role in the male reproductive tract, because the phenotype of mice lacking this isoform includes testicular atrophy and increased sperm DNA damage(PMID:20864641). It is initially synthesized as a membrane-binding 31-kDa protein and processed into a 27-kDa secretory form and is discarded with the residual bodies(PMID:19208552). PRDX4 is a pentamer of dimers(PMID:23025503).

Publications

Species	Application	Title
human	WB	Nat Commun; The deubiquitinase OTUD1 regulates immunoglobulin production and proteasome inhibitor sensitivity in multiple myeloma; Authors - Alexander Vdovin ; KD Validated
mouse	IF	Biochem Biophys Res Commun; Peroxiredoxin 4 secreted by cumulus cells ameliorates the maturation of oocytes in vitro; Authors - Wangjuan Dai