Anticorps Monoclonal anti-PRDX4

• Phare
• Validé par KD/KO

PRDX4 Monoclonal Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

5B6F1

N° de cat : 60286-1-PBS

Synonymes

Antioxidant enzyme AOE372, AOE37 2, AOE37-2, EC:1.11.1.24, peroxiredoxin 4

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Galerie de données de validation

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  WB analysis of HepG2 using 60286-1-Ig (same clone as 60286-1-PBS)

  WB result of PRDX4 antibody (60286-1-Ig, 1:5000) with si-Control and si-PRDX4 transfected HepG2 cells. This data was developed using the same antibody clone with 60286-1-PBS in a different storage buffer formulation.

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  WB analysis using 60286-1-Ig (same clone as 60286-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60286-1-Ig (PRDX4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60286-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 60286-1-Ig (same clone as 60286-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 60286-1-Ig (PRDX4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60286-1-PBS in a different storage buffer formulation.

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  WB analysis of PC-12 using 60286-1-Ig (same clone as 60286-1-PBS)

  PC-12 cells were subjected to SDS PAGE followed by western blot with 60286-1-Ig (PRDX4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60286-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 60286-1-Ig (same clone as 60286-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 60286-1-Ig (PRDX4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60286-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 60286-1-Ig (same clone as 60286-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 60286-1-Ig (PRDX4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60286-1-PBS in a different storage buffer formulation.

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  WB analysis of K-562 using 60286-1-Ig (same clone as 60286-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 60286-1-Ig (PRDX4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60286-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 60286-1-Ig (same clone as 60286-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon slide using 60286-1-Ig (PRDX4 Antibody) at dilution of 1:50(unde10x lens). This data was developed using the same antibody clone with 60286-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 60286-1-Ig (same clone as 60286-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon slide using 60286-1-Ig (PRDX4 Antibody) at dilution of 1:50(under 40x lens). This data was developed using the same antibody clone with 60286-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 60286-1-Ig (same clone as 60286-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 60286-1-Ig (PRDX4 Antibody) at dilution of 1:50(under 10x lens). This data was developed using the same antibody clone with 60286-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 60286-1-Ig (same clone as 60286-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 60286-1-Ig (PRDX4 Antibody) at dilution of 1:50(under 40x lens). This data was developed using the same antibody clone with 60286-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 60286-1-Ig (same clone as 60286-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 60286-1-Ig (PRDX4 Antibody) at dilution of 1:50(under 10x lens). This data was developed using the same antibody clone with 60286-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 60286-1-Ig (same clone as 60286-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 60286-1-Ig (PRDX4 Antibody) at dilution of 1:50(under 40x lens). This data was developed using the same antibody clone with 60286-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 60286-1-Ig (same clone as 60286-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PRDX4 antibody (60286-1-Ig, Clone: 5B6F1 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60286-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP50708-1

  Cytometric bead array standard curve of MP50708-1, PRDX4 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60286-2-PBS. Detection antibody: 60286-1-PBS. Standard:Ag13401. Range: 0.391-100 ng/mL.

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Informations sur le produit

60286-1-PBS cible PRDX4 dans les applications de WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: PRDX4 Protéine recombinante Ag13401
• Nom complet: peroxiredoxin 4
• Masse moléculaire calculée: 31 kDa
• Poids moléculaire observé: 30 kDa
• Numéro d’acquisition GenBank: BC007107
• Symbole du gène: PRDX4
• Identification du gène (NCBI): 10549
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

PRDX4(Peroxiredoxin-4) is also named as AOE37-2, Prx-IV and belongs to the AhpC/TSA family. PRDX4 is associated with acrosome formation during rat spermatogenesis and has a protective role in the male reproductive tract, because the phenotype of mice lacking this isoform includes testicular atrophy and increased sperm DNA damage(PMID:20864641). It is initially synthesized as a membrane-binding 31-kDa protein and processed into a 27-kDa secretory form and is discarded with the residual bodies(PMID:19208552). PRDX4 is a pentamer of dimers(PMID:23025503).

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