Anticorps Polyclonal de lapin anti-PRDX6

• Phare
• Validé par KD/KO

PRDX6 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

26

N° de cat : 13585-1-AP

Synonymes

1 Cys, 1 Cys peroxiredoxin, 1 Cys PRX, 24 kDa protein, aiPLA2, Antioxidant protein 2, AOP2, KIAA0106, Liver 2D page spot 40, NSGPx, p29, peroxiredoxin 6, PRDX6, PRX, Red blood cells page spot 12

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Galerie de données de validation

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  WB analysis using 13585-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13585-1-AP (PRDX6 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of HAP1 using 13585-1-AP

  HAP1 (WT and PRDX6 KO) lysates prepared with RIPA buffer, 25 μg protein loaded. 13585-1-AP incubated at 1:2000 at 4°C overnight in 5% BSA in TBST. Ponceau stained transfers shown on right. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.

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  WB analysis of HeLa using 13585-1-AP

  WB result of PRDX6 antibody (13585-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PRDX6 transfected HeLa cells.

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  WB analysis using 13585-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13585-1-AP (PRDX6 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 13585-1-AP

  IP Result of anti-PRDX6 (IP:13585-1-AP, 3ug; Detection:13585-1-AP 1:500) with HeLa cells lysate 2800ug.

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  IP experiment of HAP1 using 13585-1-AP

  HAP1 lysates prepared and IP of peroxidoxin 6 performed using 2.0 μg of 13585-1-AP coupled to protein A- Sepharose beads. Ponceau stained transfers shown for each blot. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.

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  IHC staining of human brain using 13585-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 13585-1-AP (PRDX6 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human brain using 13585-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 13585-1-AP (PRDX6 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human brain using 13585-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 13585-1-AP (PRDX6 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human brain using 13585-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 13585-1-AP (PRDX6 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 13585-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 13585-1-AP (PRDX6 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HAP1 using 13585-1-AP

  HAP1 WT cells (yellow outline) and PRDX6 KO cells (red outline) labelled with a green or a far red fluorescence dye, respectively. Cells fixed with 4% PFA and stained with 13585-1-AP at 1:300 plus DAPI. Bars = 10 μm. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.

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Applications testées

• Résultats positifs en WB: cellules SH-SY5Y, cellules A549, cellules HeLa, cellules HepG2, cellules K-562, tissu hépatique de rat, tissu hépatique de souris
• Résultats positifs en IP: cellules HeLa, HAP1 cells
• Résultats positifs en IHC: tissu cérébral humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa, HAP1 cells

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 5 publications below
• WB: See 24 publications below
• IHC: See 7 publications below
• IF: See 3 publications below

Informations sur le produit

13585-1-AP cible PRDX6 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PRDX6 Protéine recombinante Ag4512
• Nom complet: peroxiredoxin 6
• Masse moléculaire calculée: 224 aa, 25 kDa
• Poids moléculaire observé: 25-30 kDa
• Numéro d’acquisition GenBank: BC035857
• Symbole du gène: PRDX6
• Identification du gène (NCBI): 9588
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PRDX6 (Peroxiredoxin-6), also named as AOP2 or KIAA0106, is a unique member of the peroxiredoxin family of antioxidants. PRDX6 is highly expressed in liver and protects cells from oxidative damage by reducing H2O2 and various lipid Peroxides (PMID: 17382207). It can form a dimer(PMID:20500660).PRDX6 is expressed in all major organs, with a particularly high level in lung (PMID:15890616). Prdx6 is detected at approximately 24 to 28 kDa, and can be monosumoylated with the molecular mass of about 40 kDa (PMID: 24910119).

Publications

Species	Application	Title
human	WB	Nat Commun; Suppression of mitochondrial ROS by prohibitin drives glioblastoma progression and therapeutic resistance.; Authors - Haohao Huang
human	WB	Nat Commun; Functional screening implicates miR-371-3p and peroxiredoxin 6 in reversible tolerance to cancer drugs.; Authors - Nisebita Sahu ; KO Validated
rat	WB	Br J Pharmacol; Luteolin ameliorates rat myocardial ischemia-reperfusion injury through peroxiredoxin II activation.; Authors - Bo Wei
human	WB	Antioxid Redox Signal; Peroxiredoxin 6 is a crucial factor in the initial step of mitochondrial clearance and is upstream of the PINK1-Parkin pathway.; Authors - Shuaipeng Ma
rat	WB,IHC	Environ Sci Pollut Res Int; Curcumin strengthens a spontaneous self-protective mechanism-SP1/PRDX6 pathway, against di-n-butyl phthalate-induced testicular ferroptosis damage; Authors - Hengtao Bu
human	WB	Environ Toxicol Pharmacol; 4-tert-octylphenol injures motility and viability of human sperm by affecting cAMP-PKA/PKC-tyrosine phosphorylation signals.; Authors - Shaoping Huang