Anticorps Monoclonal anti-PRDX6

• Phare
• Validé par KD/KO

PRDX6 Monoclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, porc

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

CloneNo.

3C12D3

N° de cat : 67499-1-Ig

Synonymes

1-Cys peroxiredoxin, 1-Cys PRX, 3C12D3, Acidic calcium-independent phospholipase A2, AOP2

---

Galerie de données de validation

• 

  WB analysis using 67499-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67499-1-Ig (PRDX6 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

• 

  WB analysis of HAP1 and HeLa using 67499-1-Ig

  HAP1 and HeLa (WT and PRDX6 KO) lysates prepared with RIPA buffer, 25ug protein loaded. 67499-1-Ig incubated at 1:1000 at 4°C overnight in 5% BSA in TBST. Ponceau stained transfers shown on right. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.

• 

  WB analysis of HeLa using 67499-1-Ig

  WB result of PRDX6 antibody (67499-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PRDX6 transfected HeLa cells.

• 

  WB analysis using 67499-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67499-1-Ig (PRDX6 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HAP1 using 67499-1-Ig

  HAP1 lysates prepared and IP of peroxidoxin 6 performed using 2.0 μg of 67499-1-Ig coupled to protein G- Sepharose beads. Ponceau stained transfers shown for each blot. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.

• 

  IHC staining of human liver using 67499-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67499-1-Ig (PRDX6 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 67499-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67499-1-Ig (PRDX6 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 67499-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PRDX6 antibody (67499-1-Ig, Clone: 3C12D3 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red).

• 

  IF Staining of HAP1 using 67499-1-Ig

  HAP1 WT cells (yellow outline) and PRDX6 KO cells (red outline) labelled with a green or a far red fluorescence dye, respectively. Cells fixed with 4% PFA and stained with 67499-1-lg at 1:1000 plus DAPI. Bars = 10 μm. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.

---

Applications testées

• Résultats positifs en WB: cellules LNCaP, cellules A549, cellules HEK-293, cellules HeLa, cellules HepG2, cellules Jurkat, cellules K-562, tissu cérébral de porc
• Résultats positifs en IP: HAP1 cells
• Résultats positifs en IHC: tissu hépatique humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa, HAP1

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

67499-1-Ig cible PRDX6 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, porc

• Réactivité: Humain, porc
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: PRDX6 Protéine recombinante Ag4727
• Nom complet: peroxiredoxin 6
• Masse moléculaire calculée: 224 aa, 25 kDa
• Poids moléculaire observé: 25-30 kDa
• Numéro d’acquisition GenBank: BC035857
• Symbole du gène: PRDX6
• Identification du gène (NCBI): 9588
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PRDX6 (Peroxiredoxin-6), also named as AOP2 or KIAA0106, is a unique member of the peroxiredoxin family of antioxidants. PRDX6 is highly expressed in liver and protects cells from oxidative damage by reducing H2O2 and various lipid Peroxides (PMID: 17382207). It can form a dimer(PMID:20500660).PRDX6 is expressed in all major organs, with a particularly high level in lung (PMID:15890616). Prdx6 is detected at approximately 24 to 28 kDa, and can be monosumoylated with the molecular mass of about 40 kDa (PMID: 24910119).

{{ptg:RelatedPrimaryAntibodies}}