Anticorps Recombinant de lapin anti-PRDX6

PRDX6 Recombinant Antibody for WB, Sandwich ELISA, Indirect ELISA, Sample test

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, Sandwich ELISA, Indirect ELISA, Sample test

Conjugaison

Non conjugué

CloneNo.

251053E8

N° de cat : 86368-1-PBS

Synonymes

1-Cys peroxiredoxin, 1-Cys PRX, Acidic calcium-independent phospholipase A2, AOP2, EC:1.11.1.27

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Galerie de données de validation

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  WB analysis using 86368-1-RR (same clone as 86368-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86368-1-RR (PRDX6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86368-1-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP02551-1

  Sandwich ELISA standard curve of MP02551-1, Human PRDX6 Recombinant Matched Antibody Pair - PBS only. 86368-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag4512. 86368-3-PBS was HRP conjugated as the detection antibody. Range: 0.156-10 ng/mL

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  Sample test of MP02551-1

  Plasma of six individual healthy human donors was measured. The human PRDX6 concentration of detected samples was determined to be 0.80 ng/mL with a range of 0.27 - 1.25 ng/mL.

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  Affinity and Kinetic Characterization of 86368-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86368-1-RR against Human PRDX6 were performed. The affinity constant is 0.234 nM.

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Informations sur le produit

86368-1-PBS cible PRDX6 dans les applications de WB, Sandwich ELISA, Indirect ELISA, Sample test et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: PRDX6 Protéine recombinante Ag4512
• Nom complet: peroxiredoxin 6
• Masse moléculaire calculée: 224 aa, 25 kDa
• Poids moléculaire observé: 25-28 kDa
• Numéro d’acquisition GenBank: BC035857
• Symbole du gène: PRDX6
• Identification du gène (NCBI): 9588
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

PRDX6 (Peroxiredoxin-6), also named as AOP2 or KIAA0106, is a unique member of the peroxiredoxin family of antioxidants. PRDX6 is highly expressed in liver and protects cells from oxidative damage by reducing H2O2 and various lipid Peroxides (PMID: 17382207). It can form a dimer(PMID:20500660).PRDX6 is expressed in all major organs, with a particularly high level in lung (PMID:15890616). Prdx6 is detected at approximately 24 to 28 kDa, and can be monosumoylated with the molecular mass of about 40 kDa (PMID: 24910119).

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