Anticorps Monoclonal anti-PREB

PREB Monoclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2F6A1

N° de cat : 66981-1-PBS

Synonymes

2F6A1, Guanine nucleotide-exchange factor SEC12, Prolactin regulatory element-binding protein, SEC12

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Galerie de données de validation

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  WB analysis of Daudi using 66981-1-Ig (same clone as 66981-1-PBS)

  Daudi cells were subjected to SDS PAGE followed by western blot with 66981-1-Ig (PREB antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66981-1-PBS in a different storage buffer formulation.

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  WB analysis of Ramos using 66981-1-Ig (same clone as 66981-1-PBS)

  Ramos cells were subjected to SDS PAGE followed by western blot with 66981-1-Ig (PREB antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66981-1-PBS in a different storage buffer formulation.

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  WB analysis of Raji using 66981-1-Ig (same clone as 66981-1-PBS)

  Raji cells were subjected to SDS PAGE followed by western blot with 66981-1-Ig (PREB antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66981-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 66981-1-Ig (same clone as 66981-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66981-1-Ig (PREB antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66981-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 66981-1-Ig (same clone as 66981-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66981-1-Ig (PREB antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66981-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66981-1-PBS cible PREB dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: PREB Protéine recombinante Ag28549
• Nom complet: prolactin regulatory element binding
• Masse moléculaire calculée: 45 kDa
• Poids moléculaire observé: 45 kDa
• Numéro d’acquisition GenBank: BC002765
• Symbole du gène: PREB
• Identification du gène (NCBI): 10113
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

PREB contains two PQ-rich potential transactivation domains, but no apparent DNA-binding motif, and exhibits sequence-specific binding to Pit1-binding element of the prolactin (PRL) promoter. It may act as a transcriptional regulator and is thought to be involved in some of the developmental abnormalities observed in patients with partial trisomy 2p.

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