Anticorps Polyclonal de lapin anti-PREPL

PREPL Polyclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 12478-1-PBS

Synonymes

EC:3.4.21.-, KIAA0436, PPCEL, prolyl endopeptidase like, Prolyl endopeptidase-like

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Galerie de données de validation

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  WB analysis of human brain using 12478-1-AP (same clone as 12478-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 12478-1-AP (PREPL antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12478-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 12478-1-AP (same clone as 12478-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12478-1-AP (PREPL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12478-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 12478-1-AP (same clone as 12478-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12478-1-AP (PREPL antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12478-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 12478-1-AP (same clone as 12478-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12478-1-AP (PREPL antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12478-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat brain using 12478-1-AP (same clone as 12478-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 12478-1-AP (PREPL antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12478-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat brain using 12478-1-AP (same clone as 12478-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 12478-1-AP (PREPL antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12478-1-PBS in a different storage buffer formulation.

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Informations sur le produit

12478-1-PBS cible PREPL dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PREPL Protéine recombinante Ag3196
• Nom complet: prolyl endopeptidase-like
• Masse moléculaire calculée: 72 kDa
• Poids moléculaire observé: 72 kDa
• Numéro d’acquisition GenBank: BC013193
• Symbole du gène: PREPL
• Identification du gène (NCBI): 9581
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

PREPL (Prolyl endopeptidase-like) is also named as KIAA0436 and belongs to the prolyl oligopeptidase subfamily of serine peptidases. IPREPL is a novel oligopeptidase with unique structural and functional characteristics (PMID:16385448). It has 4 isoforms produced by alternative splicing and can exsit as a homodimer (PMID:16143824). Defects in PREPL are a cause of hypotonia-cystinuria syndrome (HCS).

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