Anticorps Polyclonal de lapin anti-PREX1

PREX1 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 33193-1-PBS

Synonymes

KIAA1415, P REX1, Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 1 protein, P-Rex1, PtdIns(3,4,5)-dependent Rac exchanger 1

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Galerie de données de validation

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  WB analysis using 33193-1-AP (same clone as 33193-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 33193-1-AP (PREX1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 33193-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 33193-1-AP (same clone as 33193-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using PREX1 antibody (33193-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 33193-1-PBS in a different storage buffer formulation.

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Informations sur le produit

33193-1-PBS cible PREX1 dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PREX1 Protéine recombinante Ag36649
• Nom complet: phosphatidylinositol-3,4,5-trisphosphate-dependent Rac exchange factor 1
• Masse moléculaire calculée: 186 kDa
• Poids moléculaire observé: 186 kDa
• Numéro d’acquisition GenBank: NM_020820.3
• Symbole du gène: PREX1
• Identification du gène (NCBI): 57580
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 1 protein (PREX1) is a guanine nucleotide exchange factor (GEF) that specifically activates Rac GTPases, particularly Rac1 (PMID: 16243035). PREX1 is activated downstream of G protein-coupled receptors (GPCRs) and receptor tyrosine kinases (RTKs), such as ErbB receptors, and is involved in PI3K/AKT and MAPK signaling pathways (PMID: 28710285; 27358402). It contributes to glucose uptake in adipocytes and promotes tumor cell proliferation, migration, and metastasis in various cancers, including breast, prostate, and melanoma (PMID: 22109529).

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