Anticorps Polyclonal de lapin anti-PRICKLE1

• Phare
• Validé par KD/KO

PRICKLE1 Polyclonal Antibody for WB, IHC, IF-P, FC (Intra), IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF-P, FC (Intra), IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 22589-1-PBS

Synonymes

RILP, REST/NRSF-interacting LIM domain protein 1, Prickle-like protein 1, EPM1B

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Galerie de données de validation

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  WB analysis of mouse brain using 22589-1-AP (same clone as 22589-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 22589-1-AP (PRICKLE1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22589-1-PBS in a different storage buffer formulation.

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  WB analysis of Neuro-2a using 22589-1-AP (same clone as 22589-1-PBS)

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 22589-1-AP (PRICKLE1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22589-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse brain using 22589-1-AP (same clone as 22589-1-PBS)

  IP result of anti-PRICKLE1 (IP:22589-1-AP, 3ug; Detection:22589-1-AP 1:1000) with mouse brain tissue lysate 7000ug. This data was developed using the same antibody clone with 22589-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse cerebellum using 22589-1-AP (same clone as 22589-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 22589-1-AP (PRICKLE1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22589-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse cerebellum using 22589-1-AP (same clone as 22589-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 22589-1-AP (PRICKLE1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22589-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse cerebellum using 22589-1-AP (same clone as 22589-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using PRICKLE1 antibody (22589-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 22589-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse cerebellum using 22589-1-AP (same clone as 22589-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using PRICKLE1 antibody (22589-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 22589-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 22589-1-AP (same clone as 22589-1-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.8 ug Anti-Human PRICKLE1 (22589-1-AP)(red), or 0.8 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 22589-1-PBS in a different storage buffer formulation.

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Informations sur le produit

22589-1-PBS cible PRICKLE1 dans les applications de WB, IHC, IF-P, FC (Intra), IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PRICKLE1 Protéine recombinante Ag18407
• Nom complet: prickle homolog 1 (Drosophila)
• Masse moléculaire calculée: 831 aa, 94 kDa
• Poids moléculaire observé: 94 kDa, 55-60 kDa
• Numéro d’acquisition GenBank: BC114939
• Symbole du gène: PRICKLE1
• Identification du gène (NCBI): 144165
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purifié par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

PRICKLE1, also known as RILP, EPM1B, encodes a cytoplasmic protein with an N-terminal PET (Pk, Espinas, Testin) domain, three LIM domains and a C-terminal PKH (prickle homologous) domain. PRICKLE1 is expressed at the highest levels in placenta and lower levels in lung, liver, kidney and pancreas(PMID: 12525887). PRICKLE1 localizes to the nuclear membrane and has been implicated in the nuclear trafficking of the transcription repressors REST/NRSF and REST4. Mutations in PRICKLE1 have been linked to progressive myoclonus epilepsy(PMID: 18976727).

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