Anticorps Recombinant de lapin anti-PRICKLE1

PRICKLE1 Recombinant Antibody for WB, IHC, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

242506A8

N° de cat : 84926-1-PBS

Synonymes

242506A8, EPM1B, Prickle-like protein 1, REST/NRSF-interacting LIM domain protein 1, RILP

---

Galerie de données de validation

• 

  WB analysis of MDA-MB-231 using 84926-1-RR (same clone as 84926-1-PBS)

  MDA-MB-231 cells were subjected to SDS PAGE followed by western blot with 84926-1-RR (PRICKLE1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84926-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse cerebellum using 84926-1-RR (same clone as 84926-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 84926-1-RR (PRICKLE1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84926-1-PBS in a different storage buffer formulation.

• 

  Cytometric bead array standard curve of MP01670-2

  Cytometric bead array standard curve of MP01670-2, PRICKLE1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84926-3-PBS. Detection antibody: 84926-1-PBS. Standard: Ag18407. Range: 0.156-20 ng/mL.

• 

  Affinity and Kinetic Characterization of 84926-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84926-1-RR against Human PRICKLE1 were performed. The affinity constant is 7.50 nM.

---

Informations sur le produit

84926-1-PBS cible PRICKLE1 dans les applications de WB, IHC, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: PRICKLE1 Protéine recombinante Ag18407
• Nom complet: prickle homolog 1 (Drosophila)
• Masse moléculaire calculée: 831 aa, 94 kDa
• Poids moléculaire observé: 94 kDa
• Numéro d’acquisition GenBank: BC114939
• Symbole du gène: PRICKLE1
• Identification du gène (NCBI): 144165
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

PRICKLE1, also known as RILP, EPM1B, encodes a cytoplasmic protein with an N-terminal PET (Pk, Espinas, Testin) domain, three LIM domains and a C-terminal PKH (prickle homologous) domain. PRICKLE1 is expressed at the highest levels in placenta and lower levels in lung, liver, kidney and pancreas(PMID: 12525887). PRICKLE1 localizes to the nuclear membrane and has been implicated in the nuclear trafficking of the transcription repressors REST/NRSF and REST4. Mutations in PRICKLE1 have been linked to progressive myoclonus epilepsy(PMID: 18976727).

{{ptg:RelatedPrimaryAntibodies}}