Anticorps Recombinant de lapin anti-PRIM1

PRIM1 Recombinant Antibody for WB, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

250690B8

N° de cat : 86051-1-PBS

Synonymes

DNA primase 49 kDa subunit, DNA primase small subunit, EC:2.7.7.102, p49

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Galerie de données de validation

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  WB analysis using 86051-1-RR (same clone as 86051-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86051-1-RR (PRIM1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86051-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 86051-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86051-1-RR against Human PRIM1 were performed. The affinity constant is 27.2 pM.

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Informations sur le produit

86051-1-PBS cible PRIM1 dans les applications de WB, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: PRIM1 Protéine recombinante Ag1124
• Nom complet: primase, DNA, polypeptide 1 (49kDa)
• Masse moléculaire calculée: 50 kDa
• Poids moléculaire observé: 50 kDa
• Numéro d’acquisition GenBank: BC005266
• Symbole du gène: PRIM1
• Identification du gène (NCBI): 5557
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

DNA replication in human cells is initiated by a complex apparatus containing a DNA polymerase-alpha/primase complex. Primase synthesizes oligoribonucleotides that serve as primers for the initiation of DNA synthesis. It plays a role in both the initiation of DNA replication and the synthesis of Okazaki fragments for lagging strand synthesis. PRIM1 is a subunit of this complex.

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