Anticorps Monoclonal anti-PRKACA

• Phare
• Validé par KD/KO

PRKACA Monoclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

CloneNo.

4G10D7

N° de cat : 67491-1-PBS

Synonymes

PKA C alpha, PKACA, PRKACA

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Galerie de données de validation

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  WB analysis of HeLa using 67491-1-Ig (same clone as 67491-1-PBS)

  WB result of PRKACA antibody (67491-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PRKACA transfected HeLa cells. This data was developed using the same antibody clone with 67491-1-PBS in a different storage buffer formulation.

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  WB analysis using 67491-1-Ig (same clone as 67491-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67491-1-Ig (PRKACA antibody) at dilution of 1:2300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67491-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67491-1-Ig (same clone as 67491-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67491-1-Ig (PRKACA antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67491-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67491-1-Ig (same clone as 67491-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67491-1-Ig (PRKACA antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67491-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 67491-1-Ig (same clone as 67491-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67491-1-Ig (PRKACA antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67491-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 67491-1-Ig (same clone as 67491-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67491-1-Ig (PRKACA antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67491-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67491-1-PBS cible PRKACA dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: PRKACA Protéine recombinante Ag26400
• Nom complet: protein kinase, cAMP-dependent, catalytic, alpha
• Masse moléculaire calculée: 41 kDa
• Poids moléculaire observé: 38-43 kDa
• Numéro d’acquisition GenBank: BC039846
• Symbole du gène: PRKACA
• Identification du gène (NCBI): 5566
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

PRKACA, also named as PKACA and PKA C-alpha, belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family and cAMP subfamily. It phosphorylates a large number of substrates in the cytoplasm and the nucleus.

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