Anticorps Polyclonal de lapin anti-AMPK Gamma 1

• Phare
• Validé par KD/KO

AMPK Gamma 1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

7

N° de cat : 10290-1-AP

Synonymes

AMPK Gamma 1, AMPK gamma1, AMPK subunit gamma 1, AMPKG, PRKAG1

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Galerie de données de validation

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  WB analysis using 10290-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10290-1-AP (AMPK Gamma 1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 10290-1-AP

  WB result of AMPK Gamma 1 antibody (10290-1-AP; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-AMPK Gamma 1 transfected HEK-293 cells.

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  IP experiment of K-562 using 10290-1-AP

  IP result of anti-AMPK Gamma 1(IP:10290-1-AP, 4ug; Detection:10290-1-AP 1:1000) with K-562 cells lysate 3680 ug.

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  IHC staining of human testis using 10290-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10290-1-AP (AMPK gamma 1 antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 10290-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10290-1-AP (AMPK gamma 1 antibody at dilution of 1:50 (under 40x lens).

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  IHC staining of human skeletal muscle using 10290-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 10290-1-AP (AMPK gamma 1 antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human skeletal muscle using 10290-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 10290-1-AP (AMPK gamma 1 antibody at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 10290-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 10290-1-AP (AMPK gamma 1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK-293, cellules Jurkat, cellules K-562
• Résultats positifs en IP: cellules K-562,
• Résultats positifs en IHC: tissu testiculaire humain, tissu de muscle squelettique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 7 publications below

Informations sur le produit

10290-1-AP cible AMPK Gamma 1 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: AMPK Gamma 1 Protéine recombinante Ag0302
• Nom complet: protein kinase, AMP-activated, gamma 1 non-catalytic subunit
• Masse moléculaire calculée: 38 kDa
• Poids moléculaire observé: 35-38 kDa
• Numéro d’acquisition GenBank: BC000358
• Symbole du gène: PRKAG1
• Identification du gène (NCBI): 5571
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Protein kinase, AMP-activated, gamma 1 non-catalytic subunit (PRKAG1, synonyms: AMPKG, MGC8666) is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an catalytic subunit, and non-catalytic and subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and iAMP-activated protein kinase (AMPK) is a highly conserved heterotrimeric serine/threonine kinase widely characterised as a sensor of cellular energetic stress. AMPK is a heterotrimeric complex consisting of a catalytic α-subunit and two regulatory subunits (β and γ). AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. AMPK gamma 1 is one of the gamma regulatory subunits of AMPK.

Publications

Species	Application	Title
human	WB	Cell Metab; CircACC1 Regulates Assembly and Activation of AMPK Complex under Metabolic Stress.; Authors - Qidong Li ; KD Validated
mouse	WB	JHEP Rep; Human umbilical cord mesenchymal stem cell-derived exosomes ameliorate liver steatosis by promoting fatty acid oxidation and reducing fatty acid synthesis; Authors - Fuji Yang
human	WB	J Med Chem; Identification of Pyruvate Carboxylase as the Cellular Target of Natural Bibenzyls with Potent Anticancer Activity against Hepatocellular Carcinoma via Metabolic Reprogramming.; Authors - Yuwen Sheng
human	WB	Protein Cell; AMPK interacts with DSCAM and plays an important role in netrin-1 induced neurite outgrowth.; Authors - Zhu Kun K
human	WB	J Exp Clin Cancer Res; METTL16 promotes glycolytic metabolism reprogramming and colorectal cancer progression; Authors - Wei Wei ; KD Validated
human	WB	Cell Death Dis; WW domain-binding protein 2 overexpression prevents diet-induced liver steatosis and insulin resistance through AMPKβ1.; Authors - Zhe Zheng