Anticorps Polyclonal de lapin anti-PRKAR1A

PRKAR1A Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

1

N° de cat : 20358-1-AP

Synonymes

CAR, CNC, CNC1, PKR1, PPNAD1, PRKAR1, PRKAR1A, Tissue specific extinguisher 1, TSE1

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Galerie de données de validation

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  WB analysis of HeLa using 20358-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20358-1-AP (PRKAR1A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse cerebellum using 20358-1-AP

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 20358-1-AP (PRKAR1A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 20358-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20358-1-AP (PRKAR1A antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse cerebellum using 20358-1-AP

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 20358-1-AP (PRKAR1A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 20358-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20358-1-AP (PRKAR1A antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 20358-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20358-1-AP (PRKAR1A antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 20358-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20358-1-AP (PRKAR1A antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 20358-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 20358-1-AP (PRKAR1A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, tissu de cervelet de souris
• Résultats positifs en IHC: tissu cérébral de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

20358-1-AP cible PRKAR1A dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PRKAR1A Protéine recombinante Ag14216
• Nom complet: protein kinase, cAMP-dependent, regulatory, type I, alpha (tissue specific extinguisher 1)
• Masse moléculaire calculée: 381 aa, 43 kDa
• Poids moléculaire observé: 43-45 kDa
• Numéro d’acquisition GenBank: BC036285
• Symbole du gène: PRKAR1A
• Identification du gène (NCBI): 5573
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PKA is a heterotetramer inactive kinase composed of two regulatory and two catalytic submits. The regulatory subunits are encoded by four genes (PRKAR1A, PRKAR2A, PRKAR1B, and PRKAR2B). PRKAR1A, a gene coding for the cAMP-dependent protein kinase (PKA) 1α regulatory submit, is located on human chromosome 17q22-24. PRKAR1A protein insufficiency and PKA dysregulation have been implicated in various types of disorders, including Albright hereditary osteodystrophy (AHO), pseudohypoparathyroidism (PHP), acrodysostosis (ACRDYS), and Carney complex. PRKAR1A protein expression level was significantly dysregulated in multiple primary carcinomas and distant metastases, such as cardiac myxomas, odontogenic myxomas, anaplastic thyroid carcinomas, breast cancer, pediatric pituitary adenomas, and Schwann cell tumors.

Publications

Species	Application	Title
human	WB	Mol Cell; Systematically defining selective autophagy receptor-specific cargo using autophagosome content profiling.; Authors - Susanne Zellner