Anticorps Polyclonal de lapin anti-PRKAR2A

• Phare
• Validé par KD/KO

PRKAR2A Polyclonal Antibody for WB, IHC, IF-P, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF-P, IP, ELISA

Conjugaison

Non conjugué

Publications(6)

N° de cat : 10142-2-AP

Synonymes

cAMP-dependent protein kinase type II-alpha regulatory subunit, PKR2, PRKAR2

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Galerie de données de validation

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  WB analysis using 10142-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10142-2-AP (PRKAR2A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 10142-2-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 10142-2-AP (PRKAR2A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 10142-2-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 10142-2-AP (PRKAR2A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10142-2-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10142-2-AP (PRKAR2A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 10142-2-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 10142-2-AP (PRKAR2A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 10142-2-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 10142-2-AP (PRKAR2A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 10142-2-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 10142-2-AP (PRKAR2A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa cells using 10142-2-AP

  IP result of anti-PRKAR2A (10142-2-AP for IP and Detection).

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  IP experiment of HeLa using 10142-2-AP

  IP result of anti-PRKAR2A (IP:10142-2-AP, 4ug; Detection:10142-2-AP 1:2000) with HeLa cells lysate 1320 ug.

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  IHC staining of mouse colon using 10142-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 10142-2-AP (PRKAR2A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse testis using 10142-2-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using PRKAR2A antibody (10142-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules K-562, cellules MCF-7, cellules PC-3, tissu testiculaire de souris, tissu testiculaire humain
• Résultats positifs en IP: cellules HeLa, HeLa cells
• Résultats positifs en IHC: tissu de côlon de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu testiculaire de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IHC: See 1 publications below
• IP: See 1 publications below

Informations sur le produit

10142-2-AP cible PRKAR2A dans les applications de WB, IHC, IF-P, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PRKAR2A Protéine recombinante Ag0203
• Nom complet: protein kinase, cAMP-dependent, regulatory, type II, alpha
• Masse moléculaire calculée: 43 kDa
• Poids moléculaire observé: 46-52 kDa
• Numéro d’acquisition GenBank: BC002763
• Symbole du gène: PRKAR2A
• Identification du gène (NCBI): 5576
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The effects of cAMP in most tissues and cell types are mainly modulated via protein kinase A, a heterotetrameric protein complex consisting of two regulatory and two catalytic subunits. The regulatory subunit of cAMP-dependent protein kinase (PRKAR2A) is one of the regulatory subunits and the gene is located on chromosome region 3p21.3-p21.2. The expression of PRKAR2A is tightly regulated during spermatogenesis, a significant increase in expression of this gene was also found in the human myometrium during pregnancy. This antibody got two bands about 45 kDa and 50-55 kDa in western blotting, and the 50-55kDa may be caused by phosphorylation.

Publications

Species	Application	Title
human	WB	Signal Transduct Target Ther; The AKAP12-PKA axis regulates lipid homeostasis during alcohol-associated liver disease; Authors - Chandana Thimme Gowda
human	WB	Cancer Lett; Depletion of LAMP3 enhances PKA-mediated VASP phosphorylation to suppress invasion and metastasis in esophageal squamous cell carcinoma.; Authors - Furong Huang ; KD Validated
rat	WB,IHC	Life Sci; TMT-based proteomics analysis to screen potential biomarkers of acute-phase TBI in rats.; Authors - Haochen Wang
mouse,human	WB,IP	Cell Rep; STK25 inhibits PKA signaling by phosphorylating PRKAR1A; Authors - Xiaokan Zhang
mouse	WB	J Transl Med; Nicotine restores olfactory function by activation of prok2R/Akt/FoxO3a axis in Parkinson's disease; Authors - Qinglong Guo
human	WB	J Transl Med; F-box protein FBXO21 overexpression inhibits the proliferation and metastasis of clear cell renal cell carcinoma and is closely related to the CREB pathway and tumor immune cell infiltration; Authors - Wuping Yang

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