Anticorps Polyclonal de lapin anti-PRKAR2B

PRKAR2B Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 28351-1-PBS

Synonymes

RII BETA, PRKAR2, cAMP-dependent protein kinase type II-beta regulatory subunit

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Galerie de données de validation

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  WB analysis using 28351-1-AP (same clone as 28351-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 28351-1-AP (PRKAR2B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28351-1-PBS in a different storage buffer formulation.

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  WB analysis using 28351-1-AP (same clone as 28351-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 28351-1-AP (PRKAR2B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28351-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse brain using 28351-1-AP (same clone as 28351-1-PBS)

  IP result of anti-PRKAR2B (IP:28351-1-AP, 4ug; Detection:28351-1-AP 1:1000) with mouse brain tissue lysate 1080 ug. This data was developed using the same antibody clone with 28351-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach using 28351-1-AP (same clone as 28351-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 28351-1-AP (PRKAR2B antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28351-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 28351-1-AP (same clone as 28351-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 28351-1-AP (PRKAR2B antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28351-1-PBS in a different storage buffer formulation.

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  IF Staining of Neuro-2a using 28351-1-AP (same clone as 28351-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Neuro-2a cells using PRKAR2B antibody (28351-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 28351-1-PBS in a different storage buffer formulation.

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  IF Staining of Neuro-2a using 28351-1-AP (same clone as 28351-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Neuro-2a cells using PRKAR2B antibody (28351-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 28351-1-PBS in a different storage buffer formulation.

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  IF Staining of SH-SY5Y using 28351-1-AP (same clone as 28351-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using PRKAR2B antibody (28351-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 28351-1-PBS in a different storage buffer formulation.

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  FC experiment of SH-SY5Y using 28351-1-AP (same clone as 28351-1-PBS)

  1X10^6 SH-SY5Y cells were intracellularly stained with 0.4 ug Anti-Human PRKAR2B (28351-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 28351-1-PBS in a different storage buffer formulation.

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Informations sur le produit

28351-1-PBS cible PRKAR2B dans les applications de WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PRKAR2B Protéine recombinante Ag28787
• Nom complet: protein kinase, cAMP-dependent, regulatory, type II, beta
• Masse moléculaire calculée: 418 aa, 46 kDa
• Poids moléculaire observé: 46-50 kDa
• Numéro d’acquisition GenBank: BC075800
• Symbole du gène: PRKAR2B
• Identification du gène (NCBI): 5577
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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