Anticorps Recombinant de lapin anti-PKC Alpha

PKC Alpha Recombinant Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

240926E1

N° de cat : 83840-6-PBS

Synonymes

PRKCA, 240926E1, AAG6, EC:2.7.11.13, PKC A

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Galerie de données de validation

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  WB analysis using 83840-6-RR (same clone as 83840-6-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83840-6-RR (PKC Alpha antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83840-6-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 83840-6-RR (same clone as 83840-6-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 83840-6-RR (PKC Alpha antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer(pH9). This data was developed using the same antibody clone with 83840-6-PBS in a different storage buffer formulation.

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  IF Staining of rat eye using 83840-6-RR (same clone as 83840-6-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat eye tissue using PKC Alpha antibody (83840-6-RR, Clone: 240926E1 ) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83840-6-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 83840-6-RR (same clone as 83840-6-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PRKCA antibody (83840-6-RR, Clone: 240926E1 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83840-6-PBS in a different storage buffer formulation.

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  FC experiment of HepG2 using 83840-6-RR (same clone as 83840-6-PBS)

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug PRKCA Recombinant antibody (83840-6-RR, Clone:240926E1) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83840-6-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83840-6-RR

  Biolayer interferometry (BLl) kinetic assays of 83840-6-RR against Human PRKCA were performed. The affinity constant is below 1 pM.

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Informations sur le produit

83840-6-PBS cible PKC Alpha dans les applications de WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: PKC Alpha Protéine recombinante Ag1025
• Nom complet: protein kinase C, alpha
• Poids moléculaire observé: 77 kDa
• Numéro d’acquisition GenBank: BC109274
• Symbole du gène: PKC Alpha
• Identification du gène (NCBI): 5578
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. PRKCA is one of the PKC family members. This kinase has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control. Knockout studies in mice suggest that this kinase may be a fundamental regulator of cardiac contractility and Ca(2+) handling in myocytes.

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