Anticorps Polyclonal de lapin anti-PRKCDBP

• Phare
• Validé par KD/KO

PRKCDBP Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 16250-1-PBS

Synonymes

Caveolae-associated protein 3, Cavin 3, Cavin-3, hSRBC, Protein kinase C delta-binding protein

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Galerie de données de validation

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  WB analysis of HeLa using 16250-1-AP (same clone as 16250-1-PBS)

  WB result of PRKCDBP antibody (16250-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PRKCDBP transfected HeLa cells. This data was developed using the same antibody clone with 16250-1-PBS in a different storage buffer formulation.

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  WB analysis using 16250-1-AP (same clone as 16250-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 16250-1-AP (PRKCDBP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16250-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 16250-1-AP (same clone as 16250-1-PBS)

  MCF7 cells were subjected to SDS PAGE followed by western blot with 16250-1-AP (PRKCDBP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16250-1-PBS in a different storage buffer formulation.

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  IP experiment of A549 using 16250-1-AP (same clone as 16250-1-PBS)

  IP result of anti-PRKCDBP (IP:16250-1-AP, 4ug; Detection:16250-1-AP 1:1000) with A549 cells lysate 1120 ug. This data was developed using the same antibody clone with 16250-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 16250-1-AP (same clone as 16250-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 16250-1-AP (PRKCDBP antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16250-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 16250-1-AP (same clone as 16250-1-PBS)

  Immunofluorescent analysis of MCF-7 cells, using PRKCDBP antibody 16250-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 16250-1-PBS in a different storage buffer formulation.

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Informations sur le produit

16250-1-PBS cible PRKCDBP dans les applications de WB, IHC, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PRKCDBP Protéine recombinante Ag9209
• Nom complet: protein kinase C, delta binding protein
• Masse moléculaire calculée: 261 aa, 28 kDa
• Poids moléculaire observé: 35-40 kDa
• Numéro d’acquisition GenBank: BC011585
• Symbole du gène: PRKCDBP
• Identification du gène (NCBI): 112464
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Protein kinase C delta-binding protein (PRKCDBP) is a putative tumor suppressor in which alteration has been observed in several human cancers. It is a BRCA1-interacting protein, and may also be involved in DNA damage response and participate in BRCA1-mediated tumor suppression pathways. PKCdelta binds to the C-terminal half of PRKCDBP through the regulatory domain and phosphorylates it in vitro. In COS1 cells, the phosphorylation of over-expressed PRKCDBP is stimulated by 12-O-tetradecanoylphorbol-13-acetate and further enhanced by the over-expression of PKCdelta

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