Anticorps Polyclonal de lapin anti-PRKCSH

• Phare
• Validé par KD/KO

PRKCSH Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 12148-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 12148-1-AP (same clone as 12148-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 12148-1-AP (PRKCSH antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12148-1-PBS in a different storage buffer formulation.

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  WB analysis using 12148-1-AP (same clone as 12148-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 12148-1-AP (PRKCSH antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12148-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 12148-1-AP (same clone as 12148-1-PBS)

  IP result of anti-PRKCSH (IP:12148-1-AP, 4ug; Detection:12148-1-AP 1:1000) with HeLa cells lysate 2000ug. This data was developed using the same antibody clone with 12148-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 12148-1-AP (same clone as 12148-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12148-1-AP (PRKCSH antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12148-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 12148-1-AP (same clone as 12148-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12148-1-AP (PRKCSH antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12148-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 12148-1-AP (same clone as 12148-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 12148-1-AP (PRKCSH antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12148-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 12148-1-AP (same clone as 12148-1-PBS)

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 12148-1-AP (PRKCSH antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12148-1-PBS in a different storage buffer formulation.

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Informations sur le produit

12148-1-PBS cible PRKCSH dans les applications de WB, IHC, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PRKCSH Protéine recombinante Ag2796
• Nom complet: protein kinase C substrate 80K-H
• Masse moléculaire calculée: 80 kDa
• Poids moléculaire observé: 80 kDa
• Numéro d’acquisition GenBank: BC013586
• Symbole du gène: PRKCSH
• Identification du gène (NCBI): 5589
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

PRKCSH encodes the beta-subunit of glucosidase II, an N-linked glycan-processing enzyme in the endoplasmic reticulum (ER). This protein is an acidic phospho-protein known to be a substrate for protein kinase C. Defects in PRKCSH are a cause of an autosomal dominant polycystic liver disease (PCLD). Glucosidase II is an ER-localized enzyme that contains α and β subunits (Glucosidase IIα and Glucosidase IIβ) which form a defined heterodimeric complex.

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