Anticorps Recombinant de lapin anti-PRKG1

PRKG1 Recombinant Antibody for WB, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

250348B5

N° de cat : 85983-1-PBS

Synonymes

CGKI, cGK1, cGMP-dependent protein kinase 1, cGMP-dependent protein kinase I, EC:2.7.11.12

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Galerie de données de validation

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  WB analysis using 85983-1-RR (same clone as 85983-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85983-1-RR (PRKG1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85983-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 85983-1-RR

  Biolayer interferometry (BLl) kinetic assays of 85983-1-RR against Human PRKG1 were performed. The affinity constant is below 1 pM.

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Informations sur le produit

85983-1-PBS cible PRKG1 dans les applications de WB, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: PRKG1 Protéine recombinante Ag16333
• Nom complet: protein kinase, cGMP-dependent, type I
• Masse moléculaire calculée: 686 aa, 78 kDa
• Poids moléculaire observé: 65-78 kDa
• Numéro d’acquisition GenBank: BC127090
• Symbole du gène: PRKG1
• Identification du gène (NCBI): 5592
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

PRKG1(cGMP-dependent protein kinase 1) is also named as cGK1, PRKG1B, PRKGR1A, PRKGR1B and belongs to the cGMP subfamily. It serves as an integral component of second messenger signaling in a number of biological contexts including cell differentiation, memory, and vasodilation (PMID:21893290). Its activity prevents pathological-level nitric oxide-induced apoptosis and promotes DNA synthesis/cell proliferation in vascular smooth muscle cells (PMID:20060325). PRKG1 has 2 isoforms produced by alternative splicing with the molecular weight of 76 kDa and 78 kDa. The autophosphorylation can increase the kinase activity and it can form a monomer with the molecular weight of 65 kDa that is produced by proteolytic cleavage. SDS-PAGE revealed that proteolysis generated two different monomers with molecular masses of 70 and 68 kDa of CGK1-beta(PMID:8702828).

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