Anticorps Recombinant de lapin anti-PRKG1

PRKG1 Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

250348B5

N° de cat : 85983-1-RR

Synonymes

CGKI, cGK1, cGMP-dependent protein kinase 1, cGMP-dependent protein kinase I, EC:2.7.11.12

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Galerie de données de validation

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  WB analysis using 85983-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85983-1-RR (PRKG1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 85983-1-RR

  Biolayer interferometry (BLl) kinetic assays of 85983-1-RR against Human PRKG1 were performed. The affinity constant is below 1 pM.

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Applications testées

• Résultats positifs en WB: tissu rénal de souris, tissu cérébral de souris, tissu pulmonaire de rat, tissu pulmonaire de souris

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

85983-1-RR cible PRKG1 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: PRKG1 Protéine recombinante Ag16333
• Nom complet: protein kinase, cGMP-dependent, type I
• Masse moléculaire calculée: 686 aa, 78 kDa
• Poids moléculaire observé: 65-78 kDa
• Numéro d’acquisition GenBank: BC127090
• Symbole du gène: PRKG1
• Identification du gène (NCBI): 5592
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PRKG1(cGMP-dependent protein kinase 1) is also named as cGK1, PRKG1B, PRKGR1A, PRKGR1B and belongs to the cGMP subfamily. It serves as an integral component of second messenger signaling in a number of biological contexts including cell differentiation, memory, and vasodilation (PMID:21893290). Its activity prevents pathological-level nitric oxide-induced apoptosis and promotes DNA synthesis/cell proliferation in vascular smooth muscle cells (PMID:20060325). PRKG1 has 2 isoforms produced by alternative splicing with the molecular weight of 76 kDa and 78 kDa. The autophosphorylation can increase the kinase activity and it can form a monomer with the molecular weight of 65 kDa that is produced by proteolytic cleavage. SDS-PAGE revealed that proteolysis generated two different monomers with molecular masses of 70 and 68 kDa of CGK1-beta(PMID:8702828).

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