Anticorps Monoclonal anti-PRKRA

PRKRA Monoclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1D4C3

N° de cat : 68518-1-PBS

Synonymes

PACT, Interferon-inducible double-stranded RNA-dependent protein kinase activator A, HSD-14, HSD14, HSD 14

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Galerie de données de validation

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  WB analysis using 68518-1-Ig (same clone as 68518-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68518-1-Ig (PRKRA antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68518-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 68518-1-Ig (same clone as 68518-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 68518-1-Ig (PRKRA antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68518-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 68518-1-Ig (same clone as 68518-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 68518-1-Ig (PRKRA antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68518-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 68518-1-Ig (same clone as 68518-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68518-1-Ig (PRKRA antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68518-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 68518-1-Ig (same clone as 68518-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68518-1-Ig (PRKRA antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68518-1-PBS in a different storage buffer formulation.

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  IHC staining of rat brain using 68518-1-Ig (same clone as 68518-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 68518-1-Ig (PRKRA antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68518-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat brain using 68518-1-Ig (same clone as 68518-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 68518-1-Ig (PRKRA antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68518-1-PBS in a different storage buffer formulation.

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  IHC staining of rat cerebellum using 68518-1-Ig (same clone as 68518-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 68518-1-Ig (PRKRA antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68518-1-PBS in a different storage buffer formulation.

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Informations sur le produit

68518-1-PBS cible PRKRA dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: PRKRA Protéine recombinante Ag29818
• Nom complet: protein kinase, interferon-inducible double stranded RNA dependent activator
• Masse moléculaire calculée: 34 kDa
• Poids moléculaire observé: 34 kDa
• Numéro d’acquisition GenBank: BC009470
• Symbole du gène: PRKRA
• Identification du gène (NCBI): 8575
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

PRKRA (PKR-associated protein X) is also named as PACT, RAX, HSD-14 and HSD14. PRKRA was significantly overexpressed in PC tissues and promoted the proliferation, migration, and chemoresistance of PC cells (PMID: 37484321). PRKRA activates EIF2AK2/PKR in the absence of double-stranded RNA (dsRNA), leading to phosphorylation of EIF2S1/EFI2-alpha and inhibition of translation and induction of apoptosis. PRKRA has been shown to interact with DICER1. DICER1 and PRKRA expression in normal colon was limited to mucosal epithelium and showed similar cytoplasmic expression (PMID: 37484321).

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