Anticorps Polyclonal de lapin anti-PRKRIP1

PRKRIP1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 16794-1-PBS

Synonymes

C114, PRKR interacting protein 1, PRKR-interacting protein 1

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Galerie de données de validation

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  WB analysis using 16794-1-AP (same clone as 16794-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 16794-1-AP (PRKRIP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16794-1-PBS in a different storage buffer formulation.

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  IP experiment of NIH/3T3 using 16794-1-AP (same clone as 16794-1-PBS)

  IP result of anti-PRKRIP1 (IP:16794-1-AP, 4ug; Detection:16794-1-AP 1:2000) with NIH/3T3 cells lysate 1200 ug. This data was developed using the same antibody clone with 16794-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 16794-1-AP (same clone as 16794-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16794-1-AP (PRKRIP1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16794-1-PBS in a different storage buffer formulation.

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  IF Staining of Caco-2 using 16794-1-AP (same clone as 16794-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using PRKRIP1 antibody (16794-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 16794-1-PBS in a different storage buffer formulation.

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Informations sur le produit

16794-1-PBS cible PRKRIP1 dans les applications de WB, IHC, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PRKRIP1 Protéine recombinante Ag10266
• Nom complet: PRKR interacting protein 1 (IL11 inducible)
• Masse moléculaire calculée: 184 aa, 21 kDa
• Poids moléculaire observé: 21-25 kDa
• Numéro d’acquisition GenBank: BC014298
• Symbole du gène: PRKRIP1
• Identification du gène (NCBI): 79706
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

PRKR-interacting protein 1 (PRKRIP1), a component of the splicing C complex, is a double-stranded RNA-binding protein. PRKRIP1 consists of a fully extended N-terminal loop (residues 51-75) and an 18-turn α helix (residues 76-142) that spans 100 Å (PMID: 28502770). The full-length cDNA contains an open reading frame of 561 bp, which encodes an 184-amino acid polypeptide with a predicted molecular mass of 21 kDa (PMID: 12679338).

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