Anticorps Monoclonal anti-PRLR

PRLR Monoclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2E10G5

N° de cat : 67292-1-PBS

Synonymes

2E10G5, hPRLrI, PRL R, PRL-R, prolactin receptor

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Galerie de données de validation

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  WB analysis using 67292-1-Ig (same clone as 67292-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67292-1-Ig (PRLR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67292-1-PBS in a different storage buffer formulation.

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  WB analysis of A431 using 67292-1-Ig (same clone as 67292-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 67292-1-Ig (PRLR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67292-1-PBS in a different storage buffer formulation.

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  WB analysis of T-47D using 67292-1-Ig (same clone as 67292-1-PBS)

  T-47D cells were subjected to SDS PAGE followed by western blot with 67292-1-Ig (PRLR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67292-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67292-1-Ig (same clone as 67292-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67292-1-Ig (PRLR antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67292-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67292-1-Ig (same clone as 67292-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67292-1-Ig (PRLR antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67292-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67292-1-PBS cible PRLR dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: PRLR Protéine recombinante Ag28778
• Nom complet: prolactin receptor
• Masse moléculaire calculée: 622 aa, 70 kDa
• Poids moléculaire observé: 45 kDa, 70 kDa, 85-95 kDa
• Numéro d’acquisition GenBank: BC059392
• Symbole du gène: PRLR
• Identification du gène (NCBI): 5618
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

PRLR belongs to the cytokine type I subfamily, which also includes receptors for leptin, leukemia inhibiting factor, erythropoietin and so on. PRLR has long and short forms at approximately 70 and 45 kDa, respectively, and the mature, glycosylated form of PRLR migrates at 85-95 kDa in human decidua and placenta (PMID: 18258683).

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