Anticorps Monoclonal anti-PRMT2

PRMT2 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1F1B9

N° de cat : 66885-1-PBS

Synonymes

HMT1, HRMT1L1, PRMT2

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Galerie de données de validation

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  WB analysis of SKOV-3 using 66885-1-Ig (same clone as 66885-1-PBS)

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 66885-1-Ig (PRMT2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66885-1-PBS in a different storage buffer formulation.

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  WB analysis of rat heart using 66885-1-Ig (same clone as 66885-1-PBS)

  rat heart tissue were subjected to SDS PAGE followed by western blot with 66885-1-Ig (PRMT2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66885-1-PBS in a different storage buffer formulation.

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  WB analysis of LNCaP using 66885-1-Ig (same clone as 66885-1-PBS)

  LNCaP cells were subjected to SDS PAGE followed by western blot with 66885-1-Ig (PRMT2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66885-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 66885-1-Ig (same clone as 66885-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66885-1-Ig (PRMT2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66885-1-PBS in a different storage buffer formulation.

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  WB analysis of 4T1 using 66885-1-Ig (same clone as 66885-1-PBS)

  4T1 cells were subjected to SDS PAGE followed by western blot with 66885-1-Ig (PRMT2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66885-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66885-1-Ig (same clone as 66885-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66885-1-Ig (PRMT2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66885-1-PBS in a different storage buffer formulation.

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  IHC staining of human thyroid cancer using 66885-1-Ig (same clone as 66885-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 66885-1-Ig (PRMT2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66885-1-PBS in a different storage buffer formulation.

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  IHC staining of human thyroid cancer using 66885-1-Ig (same clone as 66885-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 66885-1-Ig (PRMT2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66885-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66885-1-Ig (same clone as 66885-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using PRMT2 antibody (66885-1-Ig, Clone: 1F1B9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66885-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66885-1-PBS cible PRMT2 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: PRMT2 Protéine recombinante Ag1806
• Nom complet: protein arginine methyltransferase 2
• Masse moléculaire calculée: 49 kDa
• Poids moléculaire observé: 45-50 kDa
• Numéro d’acquisition GenBank: BC000727
• Symbole du gène: PRMT2
• Identification du gène (NCBI): 3275
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

PRMT2 (also known as HRMT1L1) is a member of the arginine methyltransferase family which catalyzes arginine methylation and regulates diverse cellular processes, including transcription, translation, DNA repair, and RNA processing. Recently PRMT2 has been identified as a pro-tumorigenic factor in malignant gliomas.

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