Anticorps Recombinant de lapin anti-PRMT3

PRMT3 Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

241092E6

N° de cat : 83969-7-PBS

Synonymes

Protein arginine N-methyltransferase 3, PRMT 3, HRMT1L3, Heterogeneous nuclear ribonucleoprotein methyltransferase-like protein 3, EC:2.1.1.319

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Galerie de données de validation

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  WB analysis using 83969-7-RR (same clone as 83969-7-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83969-7-RR (PRMT3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83969-7-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 83969-7-RR (same clone as 83969-7-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PRMT3 antibody (83969-7-RR, Clone: 241092E6 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83969-7-PBS in a different storage buffer formulation.

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  FC experiment of A549 using 83969-7-RR (same clone as 83969-7-PBS)

  1x10^6 A549 cells were intracellularly stained with 0.25 ug PRMT3 Recombinant antibody (83969-7-RR, Clone:241092E6) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83969-7-PBS in a different storage buffer formulation.

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Informations sur le produit

83969-7-PBS cible PRMT3 dans les applications de WB, IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: PRMT3 Protéine recombinante Ag13466
• Nom complet: protein arginine methyltransferase 3
• Masse moléculaire calculée: 531 aa, 60 kDa
• Poids moléculaire observé: 60-68 kDa
• Numéro d’acquisition GenBank: BC037544
• Symbole du gène: PRMT3
• Identification du gène (NCBI): 10196
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

PRMT3(protein arginine methyltransferase 3) regulates protein functions by introducing asymmetric dimethylation marks at the arginine residues in proteins.

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