Anticorps Polyclonal de lapin anti-PRMT5

• Phare
• Validé par KD/KO

PRMT5 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications(23)

Avis (1)

N° de cat : 18436-1-AP

Synonymes

72 kDa ICln-binding protein, EC:2.1.1.320, Histone-arginine N-methyltransferase PRMT5, Jak-binding protein 1, Protein arginine N methyltransferase 5

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Galerie de données de validation

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  WB analysis using 18436-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18436-1-AP (PRMT5 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis using 18436-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18436-1-AP (PRMT5 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 18436-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18436-1-AP (PRMT5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 18436-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18436-1-AP (PRMT5 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse embryo using 18436-1-AP

  mouse embryo tissue were subjected to SDS PAGE followed by western blot with 18436-1-AP (PRMT5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 18436-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 18436-1-AP (PRMT5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 18436-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 18436-1-AP (PRMT5 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 18436-1-AP

  IP result of anti-PRMT5 (IP:18436-1-AP, 4ug; Detection:18436-1-AP 1:2000) with mouse brain tissue lysate 1280 ug.

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  IHC staining of mouse liver using 18436-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 18436-1-AP (PRMT5 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 18436-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 18436-1-AP (PRMT5 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 18436-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 18436-1-AP (PRMT5 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung using 18436-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 18436-1-AP (PRMT5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung using 18436-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 18436-1-AP (PRMT5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 18436-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 18436-1-AP (PRMT5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 18436-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 18436-1-AP (PRMT5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 18436-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 18436-1-AP (PRMT5 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human lung cancer using 18436-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 18436-1-AP (PRMT5 antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human lymphoma using 18436-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 18436-1-AP (PRMT5 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lymphoma using 18436-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 18436-1-AP (PRMT5 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 18436-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PRMT5 antibody (18436-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HepG2 using 18436-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PRMT5 antibody (18436-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HeLa using 18436-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human PRMT5 (18436-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug rabbit IgG isotype control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules HeLa, cellules Jurkat, cellules NIH/3T3, cellules Raji, tissu cérébral de rat, tissu cérébral de souris, tissu embryonnaire de souris
• Résultats positifs en IP: tissu cérébral de souris,
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu de cancer du côlon humain, tissu de cancer du poumon humain, tissu de lymphome humain, tissu hépatique de souris, tissu pulmonaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2, cellules HeLa
• Résultats positifs en FC (Intra): cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 9 publications below
• WB: See 22 publications below
• IHC: See 4 publications below
• IF: See 3 publications below
• IP: See 7 publications below
• CoIP: See 1 publications below

Informations sur le produit

18436-1-AP cible PRMT5 dans les applications de WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PRMT5 Protéine recombinante Ag9736
• Nom complet: protein arginine methyltransferase 5
• Masse moléculaire calculée: 637 aa, 73 kDa
• Poids moléculaire observé: 68-70 kDa
• Numéro d’acquisition GenBank: BC025979
• Symbole du gène: PRMT5
• Identification du gène (NCBI): 10419
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PRMT5 is a Type II enzyme that catalyzes arginine monomethylation and symmetric dimethylation (Rme1 and Rme2s). The many targets of PRMT5 include ribosomal proteins, the histone chaperone nucleoplasmin, p53, and histones. PRMT5 is frequently observed in a complex with the cofactor, methylosome protein 50 (MEP50), which is required for PRMT5 activity. PRMT5 is upregulated in several human malignancies, including lymphomas, lung cancer, breast cancer and colorectal cancer.

Publications

Species	Application	Title
human	WB,IP,IF	Adv Sci (Weinh); TBL2 Promotes Tumorigenesis via PRMT5/WDR77-Mediated AKT Activation in Breast Cancer; Authors - Xiuqing Lu
human	WB	Sci Adv; Arginine methyltransferase PRMT5 negatively regulates cGAS-mediated antiviral immune response.; Authors - Dapeng Ma ; KD Validated
human	WB	Nat Commun; The LINC01138 drives malignancies via activating arginine methyltransferase 5 in hepatocellular carcinoma.; Authors - Zhe Li
	IHC	Nat Commun; PRMT5-mediated arginine methylation activates AKT kinase to govern tumorigenesis.; Authors - Shasha Yin
human	WB,IHC	EMBO J; MST2 methylation by PRMT5 inhibits Hippo signaling and promotes pancreatic cancer progression; Authors - Yan Sun ; KD Validated
human	WB,IP	Nat Commun; USP9X regulates centrosome duplication and promotes breast carcinogenesis.; Authors - Xin Li

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Satyendra (Verified Customer) (07-16-2021)

I got really nice band of PRMT5 Knock out and knockdown

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK 293

PRMT5 Antibody Western Blot validation (1:1000 dilution) in HEK 293 (Cat no:18436-1-AP)

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