Anticorps Polyclonal de lapin anti-PRMT5

PRMT5 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

N° de cat : 27109-1-AP

Synonymes

72 kDa ICln-binding protein, EC:2.1.1.320, Histone-arginine N-methyltransferase PRMT5, Jak-binding protein 1, Protein arginine N methyltransferase 5

---

Galerie de données de validation

• 

  WB analysis using 27109-1-AP

  Various cells were subjected to SDS PAGE followed by western blot with 27109-1-AP (PRMT5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 27109-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27109-1-AP (PRMT5 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-12 using 27109-1-AP

  PC-12 cells were subjected to SDS PAGE followed by western blot with 27109-1-AP (PRMT5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 27109-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 27109-1-AP (PRMT5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 27109-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 27109-1-AP (PRMT5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

---

Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules HepG2, cellules HL-60, cellules NIH/3T3, cellules PC-12, cellules Raji

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

27109-1-AP cible PRMT5 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PRMT5 Protéine recombinante Ag25896
• Nom complet: protein arginine methyltransferase 5
• Masse moléculaire calculée: 637 aa, 73 kDa
• Poids moléculaire observé: 68 kDa
• Numéro d’acquisition GenBank: BC025979
• Symbole du gène: PRMT5
• Identification du gène (NCBI): 10419
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PRMT5 is a Type II enzyme that catalyzes arginine monomethylation and symmetric dimethylation (Rme1 and Rme2s). The many targets of PRMT5 include ribosomal proteins, the histone chaperone nucleoplasmin, p53, and histones. PRMT5 is frequently observed in a complex with the cofactor, methylosome protein 50 (MEP50), which is required for PRMT5 activity. PRMT5 is upregulated in several human malignancies, including lymphomas, lung cancer, breast cancer and colorectal cancer.

{{ptg:RelatedPrimaryAntibodies}}