Anticorps Recombinant de lapin anti-PRMT5

• Phare
• Validé par KD/KO

PRMT5 Recombinant Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

250553B5

N° de cat : 82750-2-PBS

Synonymes

72 kDa ICln-binding protein, EC:2.1.1.320, Histone-arginine N-methyltransferase PRMT5, Jak-binding protein 1, Protein arginine N methyltransferase 5

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Galerie de données de validation

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  WB analysis using 82750-2-RR (same clone as 82750-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82750-2-RR (PRMT5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82750-2-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 82750-2-RR (same clone as 82750-2-PBS)

  WB result of PRMT5 antibody (82750-2-RR; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PRMT5 transfected HepG2 cells. This data was developed using the same antibody clone with 82750-2-PBS in a different storage buffer formulation.

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  IP experiment of mouse brain using 82750-2-RR (same clone as 82750-2-PBS)

  IP result of anti-PRMT5 (IP:82750-2-RR, 4ug; Detection:82750-2-RR 1:3000) with mouse brain tissue lysate 1720 ug. This data was developed using the same antibody clone with 82750-2-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 82750-2-RR (same clone as 82750-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 82750-2-RR (PRMT5 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82750-2-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 82750-2-RR (same clone as 82750-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 82750-2-RR (PRMT5 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82750-2-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 82750-2-RR (same clone as 82750-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PRMT5 antibody (82750-2-RR, Clone: 250553B5 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 82750-2-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82750-2-RR

  Biolayer interferometry (BLl) kinetic assays of 82750-2-RR against Human PRMT5 were performed. The affinity constant is 0.923 nM.

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Informations sur le produit

82750-2-PBS cible PRMT5 dans les applications de WB, IHC, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: PRMT5 Protéine recombinante Ag9736
• Nom complet: protein arginine methyltransferase 5
• Masse moléculaire calculée: 637 aa, 73 kDa
• Poids moléculaire observé: 68 kDa
• Numéro d’acquisition GenBank: BC025979
• Symbole du gène: PRMT5
• Identification du gène (NCBI): 10419
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

PRMT5 is a Type II enzyme that catalyzes arginine monomethylation and symmetric dimethylation (Rme1 and Rme2s). The many targets of PRMT5 include ribosomal proteins, the histone chaperone nucleoplasmin, p53, and histones. PRMT5 is frequently observed in a complex with the cofactor, methylosome protein 50 (MEP50), which is required for PRMT5 activity. PRMT5 is upregulated in several human malignancies, including lymphomas, lung cancer, breast cancer and colorectal cancer.

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