Anticorps Monoclonal anti-PRMT6

• Phare
• Validé par KD/KO

PRMT6 Monoclonal Antibody for WB, Indirect ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, rat, souris

Applications

WB, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

4B5A9

N° de cat : 67981-1-PBS

Synonymes

4B5A9, EC:2.1.1.319, Heterogeneous nuclear ribonucleoprotein methyltransferase-like protein 6, Histone-arginine N-methyltransferase PRMT6, HRMT1L6

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Galerie de données de validation

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  WB analysis using 67981-1-Ig (same clone as 67981-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67981-1-Ig (PRMT6 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67981-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 67981-1-Ig (same clone as 67981-1-PBS)

  WB result of PRMT6 antibody (67981-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PRMT6 transfected MCF-7 cells. This data was developed using the same antibody clone with 67981-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67981-1-PBS cible PRMT6 dans les applications de WB, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: PRMT6 Protéine recombinante Ag7934
• Nom complet: protein arginine methyltransferase 6
• Masse moléculaire calculée: 42 kDa
• Poids moléculaire observé: 42 kDa
• Numéro d’acquisition GenBank: BC002729
• Symbole du gène: PRMT6
• Identification du gène (NCBI): 55170
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Protein arginine methyltransferase 6 (PRMT6) is a type I PRMT which is involved in epigenetic regulation of gene expression, alternative splicing, development and differentiation, DNA repair, cell proliferation and senescence, DNA methylation, mitosis, inflammation, innate antiviral immunity, spermatogenesis, transactivation of nuclear receptors and cell signaling. The human PRMT6 gene, located on Chromosome 1, encodes for the 41.9 kDa PRMT6 enzyme. PRMT6 is predominantly localized to the nucleus, in stark contrast to PRMT3 and PRMT5 which are preponderantly cytosolic, while other PRMTs are found in both nucleus and cytosol. PRMT6 is expressed in a wide range of tissues with high expression in kidney and testes. PRMT6 generates asymmetric dimethylation modifications in histone 3 at arginine 2, arginine 17 and arginine 42 (H3R2me2a, H3R17me2a and H3R42me2a) and in histone H2A at arginine 26 (H2AR26me2a) and participates in the epigenetic regulation of gene expression.

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