Anticorps Monoclonal anti-PRMT7

PRMT7 Monoclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, rat

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(2)

CloneNo.

3C5H6

N° de cat : 67669-1-Ig

Synonymes

FLJ10640, KIAA1933, PRMT7

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Galerie de données de validation

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  WB analysis using 67669-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67669-1-Ig (PRMT7 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HSC-T6 using 67669-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67669-1-Ig (PRMT7 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 67669-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 67669-1-Ig (PRMT7 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IF Staining of Jurkat using 67669-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using PRMT7 antibody (67669-1-Ig, Clone: 3C5H6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HepG2, cellules HSC-T6, cellules Jurkat
• Résultats positifs en IF/ICC: cellules Jurkat,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below

Informations sur le produit

67669-1-Ig cible PRMT7 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: PRMT7 Protéine recombinante Ag30434
• Nom complet: protein arginine methyltransferase 7
• Masse moléculaire calculée: 78 kDa
• Poids moléculaire observé: 78 kDa
• Numéro d’acquisition GenBank: BC000146
• Symbole du gène: PRMT7
• Identification du gène (NCBI): 54496
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Protein arginine methyltransferases (PRMTs) are responsible for arginine methylation of histone or nonhistone proteins to regulate diverse biological processes, including cell proliferation, differentiation and senescence. PRMT7 is expressed in both embryonic stem and germ cells and is implicated in maintenance of muscle stem cell function. overexpression of PRMT7 has been associated with cancer metastasis. PRMT7 automethylation at residue R531 was reported to enhance breast cancer metastasis.

Publications

Species	Application	Title
human	WB	EMBO J; MST2 methylation by PRMT5 inhibits Hippo signaling and promotes pancreatic cancer progression; Authors - Yan Sun
human	WB	Gastroenterology; Genetic Control of Alternative Splicing and its Distinct Role in Colorectal Cancer Mechanisms; Authors - Ming Zhang

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